Summary of project PR001424
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001424. The data can be accessed directly via it's Project DOI: 10.21228/M8NM68 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001424 |
| Project DOI: | doi: 10.21228/M8NM68 |
| Project Title: | U2OS Etoposide-PRDX1 metabolomics |
| Project Type: | MS quantitative analysis |
| Project Summary: | A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis. While cellular metabolism impacts the DNA damage response, a systematic understanding of the metabolic requirements that are crucial for DNA damage repair has yet to be achieved. Here, we investigate the metabolic enzymes and processes that are essential when cells are exposed to DNA damage. By integrating functional genomics with chromatin proteomics and metabolomics, we provide a detailed description of the interplay between cellular metabolism and the DNA damage response. Subsequent analysis identified Peroxiredoxin 1, PRDX1, as fundamental for DNA damage repair. During the DNA damage response, PRDX1 translocates to the nucleus where it is required to reduce DNA damage-induced nuclear reactive oxygen species levels. Moreover, PRDX1 controls aspartate availability, which is required for the DNA damage repair-induced upregulation of de novo nucleotide synthesis. Loss of PRDX1 leads to an impairment in the clearance of γΗ2ΑΧ nuclear foci, accumulation of replicative stress and cell proliferation defects, thus revealing a crucial role for PRDX1 as a DNA damage surveillance factor. |
| Institute: | CRG |
| Department: | GRSC |
| Laboratory: | Sdelci lab |
| Last Name: | Kourtis |
| First Name: | Savvas |
| Address: | Carrer del Dr. Aiguader, 88, 08003 Barcelona |
| Email: | savvas.kourtis@crg.eu |
| Phone: | 653549060 |
Summary of all studies in project PR001424
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002234 | A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis | Homo sapiens | CRG | MS | 2023-04-03 | 1 | 30 | Uploaded data (742.1M)* |
