Summary of project PR001479
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001479. The data can be accessed directly via it's Project DOI: 10.21228/M8JM7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001479 |
| Project DOI: | doi: 10.21228/M8JM7Q |
| Project Title: | Metabolomics Discovery of Aryl Hydrocarbon Receptor Activating Metabolites from the Human Microbiota |
| Project Type: | Bacteria supernatant |
| Project Summary: | The aryl hydrocarbon receptor (AhR) is a transcription factor that regulates gene expression upon activation by small molecules. It plays a significant role in the innate immune recognition of bacteria and response to exogenous molecules in the human host. By stimulating host immune cells with microbiota metabolites, the AhR signaling enables microbiota-dependent induction, training, and function of the host immune system. AhR is a potential target for developing therapeutics to treat myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), cancer, and aging-related diseases. A variety of bioactive molecules can act as AhR agonists, including the metabolites and derivatives of indole and tryptophan. However, given the ligand-binding versatility of AhR, new methods are needed to discover novel AhR agonists. Herein, we report an analytical workflow for the deep discovery of AhR agonists from the secreted metabolome of bacteria. It is efficient to involve the activity measurement in the early stages of discovering new AhR ligands. Moreover, utilization of the AhR-chaperone complex in live cells by the AhR activity assay can mitigate the need for purifying the complex and allows for the deep discovery of low-concentration activators. |
| Institute: | University of Connecticut |
| Department: | Chemistry |
| Laboratory: | Yao Lab |
| Last Name: | Tian |
| First Name: | Huidi |
| Address: | 55 N. Eagleville Road, Unit 3060, Storrs CT 06269 |
| Email: | huidi.tian@uconn.edu |
| Phone: | 8606341143 |
| Funding Source: | NIH |
Summary of all studies in project PR001479
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002306 | Metabolomics profiling of full extracts of bacterial culture supernatants. | Bacillus megaterium; Enterococcus faecium | University of Connecticut | MS* | 2024-10-04 | 1 | 6 | Uploaded data (12.1G)* |
| ST002307 | Metabolomics profiling of the secondary fractions 4 of bacterial culture supernatants. | Bacillus megaterium; Enterococcus faecium | University of Connecticut | MS* | 2024-10-04 | 1 | 12 | Uploaded data (16G)* |
