Summary of project PR001509
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001509 |
| Project DOI: | doi: 10.21228/M8N71K |
| Project Title: | Biomolecular condensates create phospholipid-enriched microenvironments |
| Project Type: | Metabolomics of in vitro condensates |
| Project Summary: | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. |
| Institute: | Cornell University |
| Department: | Department of Pharmacology |
| Laboratory: | Dr. Samie Jaffrey |
| Last Name: | Dumelie |
| First Name: | Jason |
| Address: | 1300 York Ave, LC-524, New York City, NY |
| Email: | jdumes98@gmail.com |
| Phone: | 6465690174 |
| Funding Source: | This work was supported by the National Institutes of Health grants R35NS111631 and R01CA186702 (S.R.J.); R01AR076029, R21ES032347 and R21NS118633 (Q.C.); and NIH P01 HD067244 and support from the Starr Cancer Consortium I13-0037 (S.S.G.). |
| Publications: | Under revision |
| Contributors: | Jason G. Dumelie, Qiuying Chen, Dawson Miller, Nabeel Attarwala, Steven S. Gross and Samie R. Jaffrey1 |
Summary of all studies in project PR001509
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002349 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 1) | Mus musculus | Cornell University | MS | 2023-03-01 | 2 | 39 | Uploaded data (2.7G)* |
| ST002352 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 2) | Mus musculus | Cornell University | MS | 2023-03-01 | 2 | 18 | Uploaded data (107.9M)* |
| ST002353 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 3) | Cornell University | MS | 2023-03-01 | 2 | 27 | Uploaded data (231M)* | |
| ST002744 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 4) | Mus musculus | Cornell University | MS | 2023-07-07 | 1 | 18 | Uploaded data (2.9G)* |
| ST002745 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 6) | Mus musculus | Cornell University | MS | 2023-07-07 | 1 | 27 | Uploaded data (4.9M)* |
| ST002751 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 5) | Mus musculus | Cornell University | MS | 2023-07-10 | 1 | 8 | Uploaded data (436.9M)* |
| ST002752 | Biomolecular condensates create phospholipid-enriched microenvironments (Part 7 - reversed phase experiment set 2) | Mus musculus | Cornell University | MS | 2023-07-10 | 1 | 10 | Uploaded data (149.9K)* |
