Summary of project PR001531
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001531. The data can be accessed directly via it's Project DOI: 10.21228/M8ST4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001531 |
| Project DOI: | doi: 10.21228/M8ST4T |
| Project Title: | Ruegeria pomeroyi transporter mutant substrate drawdown |
| Project Summary: | The goal of this project was to identify bacterial transporters responsible for uptake of environmentally relevant marine metabolites. We used the model marine heterotrophic bacterium Ruegeria pomeroyi DSS-3, for which an arrayed library of single gene knockout mutants has been generated by selecting isolated from a barcoded transposon mutant library (BasSeq). Knockout mutants of putative transporters were grown on minimal medium with a single substrate as sole carbon source. Mutant defect was assessed by comparing the substrate drawdown of isolated mutants to drawdown by a pooled mutant library (BarSeq), a proxy for wildtype fitness. |
| Institute: | University of Georgia |
| Laboratory: | Moran Lab, Edison Lab |
| Last Name: | Uchimiya |
| First Name: | Mario |
| Address: | 315 Riverbend Rd, Athens, GA, 30602, USA |
| Email: | mario.uchimiya@uga.edu |
| Phone: | (706) 542-8387 |
| Funding Source: | Simons Foundation (542391), NSF (OCE-2019589) |
| Contributors: | William F. Schroer |
Summary of all studies in project PR001531
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002381 | Ruegeria pomeroyi transporter mutant substrate drawdown | Ruegeria pomeroyi | University of Georgia | NMR | 2022-12-27 | 1 | 316 | Uploaded data (3.2G)* |
