Summary of project PR001547
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001547. The data can be accessed directly via it's Project DOI: 10.21228/M8QT3T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001547 |
| Project DOI: | doi: 10.21228/M8QT3T |
| Project Title: | Human primary astrocytes finger- and footprinting metabolomics indicate biochemical alterations under ayahuasca treatment |
| Project Type: | Untargetmetabomics- hilic |
| Project Summary: | Ayahuasca (Aya) is a psychotropic Amazonian beverage formulated from the combination of the Banisteriopsis caapi vine and the Psychotria viridis leaves in a water decoction. Aya is legally used in Latin American countries and used in Brazil for religious, cultural, and therapeutic purposes. Its properties constitute a bio-psycho-social-spiritual model involving effects from ß-carboline-derived alkaloids, present in the vine, and N,N-dimethyltryptamine (DMT), a tryptamine-derived alkaloid present in the leaves, which act together in the central nervous system (CNS). Few technical-scientific studies have been conducted to understand the effects of this brew in the metabolism. Therefore, this work aims to investigate an in vitro primary astrocyte lineage model by untargeted metabolomics evaluations of two cellular subfractions: secretome and intracellular content after Aya treatment, where DMT and other ß-carbolines were previously quantified. Metabolomics analysis was performed by UHPLC-MS/MS, followed by MS-Dial data processing and statistical analysis to identify metabolites and biochemical alterations related to Aya treatment. Aya doses were applied to the cell cultures considering DMT concentrations of 1, 10 and 20 µM, which are in agreement with non-toxic and toxic DMT threshold assays in primary human astrocyte cells viability. |
| Institute: | University of Campinas |
| Department: | Analytical Chemistry Department |
| Laboratory: | Laboratory of Bioanalytics and Integrated Omics (LABIOmics) |
| Last Name: | Zandonadi |
| First Name: | Flavia |
| Address: | Rua Josué de Castro, s/n - Cidade Universitária, Campinas - SP, 13083-970 |
| Email: | flazando@unicamp.br |
| Phone: | +551935213038 |
| Funding Source: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [grant number: 88882.305841/2018-01]. Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP [grant number: 2018/01525-3] and INCT de Bioanalítica [grant numbers: FAPESP 2014/50867-3 and CNPq 465389/2014-7] |
Summary of all studies in project PR001547
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002401 | Human primary astrocytes finger- and footprinting metabolomics indicate biochemical alterations under ayahuasca treatment | Homo sapiens | University of Campinas | MS* | 2024-01-16 | 1 | 36 | Uploaded data (21.4G)* |
