Summary of project PR001549
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001549. The data can be accessed directly via it's Project DOI: 10.21228/M8G99R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001549 |
| Project DOI: | doi: 10.21228/M8G99R |
| Project Title: | Spatial, temporal, and inter-subject variation of the metabolome along the human upper intestinal tract |
| Project Summary: | Most utilization of human diets occurs in the small intestine, which remains largely unstudied. Here, we used a novel non-invasive, ingestible sampling device to probe the spatiotemporal variation of upper intestinal luminal contents during routine daily digestion in 15 healthy subjects. We analyzed 274 intestinal samples and 60 corresponding stool homogenates by combining five metabolomics assays and 16S rRNA sequencing. We identified 1,909 metabolites, including sulfonolipids and novel bile acids. Stool and intestinal metabolomes differed dramatically. Food metabolites displayed known differences and trends in dietary biomarkers, unexpected increases in dicarboxylic acids along the intestinal tract, and a positive association between luminal keto acids and fruit intake. Diet-derived and microbially linked metabolites accounted for the largest inter-subject differences. Interestingly, subjects exhibited large variation in levels of bioactive fatty acid esters of hydroxy fatty acids (FAHFAs) and sulfonolipids. Two subjects who had taken antibiotics within 6 months prior to sampling showed markedly different patterns in these and other microbially related metabolites; from this variation, we identified Blautia species as most likely to be involved in FAHFA metabolism. Thus, in vivo sampling of the human small intestine under physiologic conditions can reveal links between diet, host and microbial metabolism. |
| Institute: | University of California, Davis |
| Last Name: | Folz |
| First Name: | Jake |
| Address: | 1 Shields Ave |
| Email: | jfolz@ucdavis.edu |
| Phone: | 7155636311 |
Summary of all studies in project PR001549
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002407 | Spatial, temporal, and inter-subject variation of the metabolome along the human upper intestinal tract (MS HILIC negative data) | Homo sapiens | University of California, Davis | MS | 2023-01-04 | 1 | 331 | Uploaded data (6.7G)* |
| ST002409 | Spatial, temporal, and inter-subject variation of the metabolome along the human upper intestinal tract (MS RP positive data) | Homo sapiens | University of California, Davis | MS | 2023-01-04 | 1 | 331 | Uploaded data (4G)* |
| ST002411 | Spatial, temporal, and inter-subject variation of the metabolome along the human upper intestinal tract (MS RP negative data) | Homo sapiens | University of California, Davis | MS | 2023-01-04 | 1 | 331 | Uploaded data (3.6G)* |
