Summary of project PR001580
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001580. The data can be accessed directly via it's Project DOI: 10.21228/M8G711 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001580 |
| Project DOI: | doi: 10.21228/M8G711 |
| Project Title: | APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge |
| Project Summary: | The E4 allele of Apolipoprotein E (APOE) is associated with both metabolic dysfunction and a heightened pro-inflammatory response – two findings that may be intrinsically linked through the concept of immunometabolism. Here, we combined bulk, single-cell, and spatial transcriptomics with cell-specific and spatially resolved metabolic analyses to systematically address the role of APOE across age, neuroinflammation, and AD pathology. RNAseq highlighted immunometabolic changes across the APOE4 glial transcriptome, specifically in subsets of metabolically distinct microglia enriched in the E4 brain during aging or following an inflammatory challenge. E4 microglia display increased Hif1α expression, a disrupted TCA cycle, and are inherently pro-glycolytic, while spatial transcriptomics and MALDI mass spectrometry imaging highlight an E4-specific response to amyloid that is characterized by widespread alterations in lipid metabolism. Taken together, our findings emphasize a central role for APOE in regulating microglial immunometabolism. |
| Institute: | University of Kentucky, Department of Physiology |
| Last Name: | Devanney |
| First Name: | Nicholas |
| Address: | Physiology, 760 Press Ave, Healthy Kentucky Research Bldg, Rm152, Lexington, Kentucky, 40508, USA |
| Email: | Nicholas.Devanney@uky.edu |
| Phone: | 8593238083 |
| Project Comments: | Part 2 of 3 |
Summary of all studies in project PR001580
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002450 | APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge (Part 1 of 3) | Mus musculus | University of Kentucky, Department of Physiology | MS | 2023-01-25 | 1 | 43 | Uploaded data (128.8M)* |
| ST002451 | APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge (Part 2 of 3) | Mus musculus | University of Kentucky | MS | 2023-01-25 | 1 | 30 | Uploaded data (34.8M)* |
| ST002453 | APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge (Part 3 of 3) | Mus musculus | University of Kentucky | MS | 2023-01-25 | 1 | 162 | Uploaded data (20.2G)* |
