Summary of project PR001619
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001619. The data can be accessed directly via it's Project DOI: 10.21228/M8F41P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001619 |
| Project DOI: | doi: 10.21228/M8F41P |
| Project Title: | Lactate-dependent Transcriptional Regulation Controls Mammalian Eye Morphogenesis |
| Project Summary: | Mammalian retinal metabolism favors aerobic glycolysis. However, the role of glycolytic metabolism in retinal morphogenesis remains unknown. Here we report that aerobic glycolysis is necessary for the early stages of retinal development. Taking advantage of an unbiased approach that combines the use of eye organoids and single-cell RNA sequencing we identified specific glucose transporters and glycolytic genes in retinal progenitors. Next, we determined that the optic vesicle territory of mouse embryos displays elevated levels of glycolytic activity. At the functional level, we found that removal of Glut1 (also known as Slc2a1) and Lactate dehydrogenase A (Ldha) gene activity from developing retinal progenitors arrested eye morphogenesis. Surprisingly, we uncovered that lactate-mediated upregulation of key eye-field transcription factors was controlled by the epigenetic modification of histone H3 acetylation through histone deacetylase (Hdac) activity. Our results identify a novel bioenergetic independent role of lactate as a signaling molecule necessary for mammalian eye morphogenesis. |
| Institute: | Northwestern University |
| Last Name: | TAKATA |
| First Name: | NOZOMU |
| Address: | 303 East Superior Street, 10-220, Chicago, Illinois, 60611, USA |
| Email: | nozomu.takata@northwestern.edu |
| Phone: | 13125036066 |
Summary of all studies in project PR001619
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002506 | Natural abundance of isotopic metabolite detection in mouse eye orgnaoids. | Mus musculus | Northwestern University | MS | 2023-05-01 | 1 | 3 | Uploaded data (207.1M)* |
| ST002507 | Time-course analysis of C13 labeling in mouse eye organoids. | Mus musculus | Northwestern University | MS | 2023-06-08 | 1 | 8 | Uploaded data (501.7M)* |
| ST002508 | 13C-isotopic labeling of mouse eye organoids under three conditions. | Mus musculus | Northwestern University | MS | 2023-06-01 | 1 | 9 | Uploaded data (534.4M)* |
| ST002574 (Available on 2025-01-01) | CoA species analysis from mouse ESC-derived eye organoids | Mus musculus | Northwestern University, Feinberg School of Medicine | MS | - | - | 4 | Not available |
