Summary of project PR001630
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001630. The data can be accessed directly via it's Project DOI: 10.21228/M80T52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001630 |
| Project DOI: | doi: 10.21228/M80T52 |
| Project Title: | Using Mass Spectrometry Imaging to Map Fluxes Quantitatively in the Tumor Ecosystem |
| Project Summary: | Tumors are comprised of a multitude of cell types spanning different microenvironments. Mass spectrometry imaging (MSI) has the potential to identify metabolic patterns within the tumor ecosystem and surrounding tissues, but conventional workflows have not yet fully integrated the breadth of experimental techniques in metabolomics. Here, we combine MSI, stable isotope labeling, and a spatial variant of Isotopologue Spectral Analysis to map distributions of metabolite abundances, nutrient contributions, and metabolic turnover fluxes across the brains of mice harboring GL261 glioma, a widely used model for glioblastoma. When integrated with MSI, the combination of ion mobility, Desorption Electrospray Ionization, and Matrix Assisted Laser Desorption revealed disruption in multiple anabolic pathways. De novo fatty acid synthesis flux was determined to be increased by approximately 3-fold in glioma relative to surrounding healthy tissue. Fatty acid elongation flux was elevated even higher at 8-fold and highlights the importance of elongase activity in glioma. The fluxes we examined were uniformly increased throughout the entire tumor region, revealing a high degree of metabolic homogeneity in our model of glioblastoma. |
| Institute: | Washington University in St. Louis |
| Last Name: | Stancliffe |
| First Name: | Ethan |
| Address: | 1 Brookings Dr. Campus Box 1134, St. Louis, MO 63105 |
| Email: | estancliffe@wustl.edu |
| Phone: | 3194644881 |
Summary of all studies in project PR001630
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002534 | Using Mass Spectrometry Imaging to Map Fluxes Quantitatively in the Tumor Ecosystem | Mus musculus | Washington University in St. Louis | MS | 2023-04-13 | 1 | 22 | Uploaded data (139.8M)* |
