Summary of project PR001668
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001668. The data can be accessed directly via it's Project DOI: 10.21228/M83H7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001668 |
| Project DOI: | doi: 10.21228/M83H7N |
| Project Title: | Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice |
| Project Type: | MS Untargeted Lipidomics |
| Project Summary: | Genetic association studies have linked ATP10A and closely related type IV P-type ATPases (P4-ATPases) to insulin resistance and vascular complications, such as atherosclerosis. In addition, prior studies of mice harboring large, overlapping chromosomal deletions implicated Atp10A in the development of diet-induced obesity and insulin resistance. Here, we generated gene-specific Atp10A knockout mice and show that Atp10A-/- mice fed a high-fat diet did not gain excess weight relative to wild-type littermates. However, Atp10A-/- mice displayed female-specific dyslipidemia characterized by elevated plasma triglycerides, free fatty acids and cholesterol, as well as altered VLDL and HDL properties. We also observed increased circulating levels of several sphingolipid species along with reduced levels of eicosanoids and bile acids. The Atp10A-/- mice also displayed hepatic insulin resistance without perturbations to whole-body glucose homeostasis. Thus, ATP10A has a sex-specific role in regulating plasma lipid composition and maintaining hepatic liver insulin sensitivity in mice. |
| Institute: | Vanderbilt University |
| Department: | Biological Sciences |
| Laboratory: | Graham |
| Last Name: | Graham |
| First Name: | Todd |
| Address: | 5260 Medical Research Building III BSB |
| Email: | tr.graham@Vanderbilt.Edu |
| Phone: | 615-343-1835 |
| Publications: | TBA |
Summary of all studies in project PR001668
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002696 | Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice | Mus musculus | Vanderbilt University | MS* | 2023-05-24 | 1 | 40 | Uploaded data (90.3G)* |
