Summary of project PR001683

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001683. The data can be accessed directly via it's Project DOI: 10.21228/M8599Z This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001683
Project DOI:doi: 10.21228/M8599Z
Project Title:Metabolic alteration of MCF-7 cells upon indirect exposure to E. coli secretome: A model of studying the microbiota effect on human breast tissue
Project Type:Microbiome-breast cancer microenvironment metabolomics
Project Summary:Cancer is a challenging disease that requires a comprehensive approach for effective treatment. Various bacterial species, including clostridia, bifidobacteria, and salmonellae, have been investigated in numerous animal tumor models, cell lines, and clinical trials as gene carriers for anti-cancerous genes, including tumor suppressor genes, suicide genes, or tumor-associated antigens. Therefore, they render cell cancer more sensitive to treatment, and they can be used as drug/gene delivery vehicles. E. coli, as one of the breast tissue microbiomes, secretes metabolites that could influence the metabolism of MCF-7 cells to ensure their survival. This in vitro investigation concentrated primarily on the role of E. coli secretome modulation on the MCF-7 cells metabolism. The intra- and extracellular metabolomes of the E. coli secretome and secretome exposed MCF-7 cells were profiled using the liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Secretome-exposed MCF-7 cells were compared to unexposed controls; a total of 31 and 56 metabolites were significantly altered intra- and extracellularly, respectively. The most common metabolic pathways dysregulated after exposure were aminoacyl-tRNA biosynthesis, purine metabolism, and energy metabolism. The decrease in some purine metabolites would suggest that altering nucleotide metabolism is one of the ways the bacterial secretome kills cancer cells. The maximum discrimination between the two groups was found in lactate levels, which plays a crucial role in cancer progression. The Warburg effect causes cancer tissue to have an acidic microenvironment, which impacts cancer cell metastasis and proliferation, inflammation, immune cell function, and blood vessel development; the decrease in lactate content may also be a method by which the secretome affects cancer. Finally, some microbial metabolites from bacterial secretome have shown promising anticancer effects and can be employed as innovative ways for cancer treatment, either alone or in combination with other medicines.
Institute:King Saud University
Last Name:AlMalki
First Name:Reem
Address:King Fahad road

Summary of all studies in project PR001683

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002715 Metabolic alteration of MCF-7 cells upon indirect exposure to E. coli secretome: A model of studying the microbiota effect on human breast tissue Homo sapiens King Saud University MS* 2023-06-22 1 36 Uploaded data (47G)*