Summary of project PR001694
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001694. The data can be accessed directly via it's Project DOI: 10.21228/M8R136 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001694 |
| Project DOI: | doi: 10.21228/M8R136 |
| Project Title: | Multi-Omics profiling of Candida albicans from agar plate and suspension media |
| Project Type: | LC-MS/MS |
| Project Summary: | Candida albicans is an opportunistic pathogen that is a significant challenge to healthcare facilities worldwide, commonly found in the human gastrointestinal, respiratory, and genitourinary systems. Morphological transition allows yeast cells to diffuse through bloodstream to colonize internal organs, whilst filamentous forms is related to penetration of host mucosa and epidermal surfaces. With the help of novel analytical techniques and instruments developed in the past years, which enabled accurate, simultaneous detection and quantification of proteins and metabolites. We investigated and compared the proteome and metabolome of C. albicans grown on agar plate verses suspension culture to gain insight into the different environmental adaptation and response to stress. Multi-omics (proteomics & metabolomics) analyses were performed using a high-resolution timsTOF mass spectrometer. From the findings reported in this experiment it is worth highlighting that ease of nutritional access in suspension media favours core growth metabolism and increased translation, while impeded access in solid media favours more diverse metabolic pathways. Core growth and replication machinery are enhanced in suspension media, with several terms related to protein translation and core metabolism increased in this media. In contrast, pathogenic cell wall proteins and proteins related to cell surface were increased in cells grown on solid media. |
| Institute: | University of Sharjah |
| Department: | Research institute of medical and health science |
| Laboratory: | Biomarker Discovery Group |
| Last Name: | Facility |
| First Name: | Core |
| Address: | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah, Sharjah, UAE, Sharjah, 000, United Arab Emirates |
| Email: | tims-tof@sharjah.ac.ae |
| Phone: | +971 6 5057656 |
Summary of all studies in project PR001694
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002730 | Multi-Omics profiling of Candida albicans from agar plate and suspension media | Candida albicans | University of Sharjah | MS | 2023-06-25 | 1 | 12 | Uploaded data (5.7G)* |
