Summary of project PR001704
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001704. The data can be accessed directly via it's Project DOI: 10.21228/M8FM85 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001704 |
| Project DOI: | doi: 10.21228/M8FM85 |
| Project Title: | Metabolic effect of Lamin A/C in oligodendrocyte on brain function |
| Project Type: | LC-MS/MS metabolomics of cell type specific Lmna conditional knockout and wildtype mice brains at 26 weeks |
| Project Summary: | Oligodendrocytes are specialized cells which insulate and support axons with their myelin membrane, allowing proper brain function. Here, we identify Lamin A/C (LMNA/C) as essential for transcriptional and functional stability of myelinating oligodendrocytes. We show that LMNA/C levels increase with differentiation of progenitors and that loss of Lmna in differentiated oligodendrocytes profoundly alters their chromatin accessibility and transcriptional signature. Lmna deletion in myelinating glia is compatible with normal developmental myelination. However, altered chromatin accessibility is detected in fully differentiated oligodendrocytes together with increased expression of progenitor genes and decreased levels of lipid-related transcription factors and inner mitochondrial membrane transcripts. As mice age, they start to develop myelin-thinning and progressively worsening motor phenotype. To address the metabolic effect of LMNA/C in oligodendrocyte on brain function, we carried out LC-MS/MS metabolomic study of myelinating glia cell specific Lmna conditional knockout and wildtype mice brains at 26 weeks. Each LC-MS/MS experiment was performed with 3 biological replicates and 4 technical replicates per genotype. Overall, our data identify LMNA/C as essential for maintaining the transcriptional and functional stability of myelinating oligodendrocytes. |
| Institute: | Advanced Science Research Center - CUNY |
| Department: | Neuroscience |
| Laboratory: | Casaccia lab, He lab, MALDI and MS core. |
| Last Name: | He |
| First Name: | Ye |
| Address: | 85 St. Nicholas Terrace, New York, New York, 10031, USA |
| Email: | yhe1@gc.cuny.edu |
| Phone: | 2124133182 |
| Publications: | Pruvost M, Patzig J, Yattah C, Selcen I, Hernandez M, Park HJ, Moyon S, Liu S, Morioka MS, Shopland L, Al-Dalahmah O, Bendl J, Fullard JF, Roussos P, Goldman J, He Y, Dupree JL, Casaccia P. The stability of the myelinating oligodendrocyte transcriptome is regulated by the nuclear lamina. Cell Rep. 2023 Jul 27;42(8):112848. doi: 10.1016/j.celrep.2023.112848. Epub ahead of print. PMID: 37515770. (https://www.cell.com/cell-reports/fulltext/S2211-1247(23)00859-8) |
Summary of all studies in project PR001704
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002739 | Metabolic effect of Lamin A/C in oligodendrocyte on brain function | Mus musculus | Advanced Science Research Center - CUNY | MS* | 2023-06-22 | 1 | 24 | Uploaded data (2.5G)* |
