Summary of project PR001739
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001739. The data can be accessed directly via it's Project DOI: 10.21228/M8XD9T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001739 |
| Project DOI: | doi: 10.21228/M8XD9T |
| Project Title: | Nucleotide metabolism in pancreatic cancer cells |
| Project Summary: | The Experiment analyzes the cancer cell metabolism in two pancreatic cancer cell lines, (i.e. Panc02 and KPC FC 1245) after the knockdown of the protein cytidine deaminase (CDA). Murine Panc02 and KPC FC1245 pancreatic cancer cell lines were genetically engineered using a doxycycline inducible CRISPR/Cas9 platform with a target specific gRNA for CDA and a control non-targeting NT gRNA. CDA knockdown cells show a significant decrease of intracellular uridine levels and the accumulation of intracellular cytidine. Consistent with the decrease in intracellular uridine, sgCda cells show reduced intracellular levels of UMP, UDP and UTP compared to sgNT cells while we see no change in adenine and cytosine nucleotides (i.e., AMP, ADP, ATP and CMP, CDP, CTP, respectively) or in UDP-hexose. |
| Institute: | VIB Center for Cancer Biology |
| Last Name: | Mazzone |
| First Name: | Massimiliano |
| Address: | Herestraat 49, box 912, Leuven, Flemish Brabant, 3000, Belgium |
| Email: | massimiliano.mazzone@kuleuven.be |
| Phone: | +32-16-37.32.13 |
Summary of all studies in project PR001739
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002791 | Nucleotide metabolism in pancreatic cancer cells | Mus musculus | VIB-KU Leuven | MS | 2024-01-24 | 1 | 16 | Uploaded data (791.9M)* |
