Summary of project PR001770
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001770. The data can be accessed directly via it's Project DOI: 10.21228/M8XB0Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001770 |
| Project DOI: | doi: 10.21228/M8XB0Q |
| Project Title: | Role of the Afferent Lymph as an Immunological Conduit to Analyze Tissue Antigenic and Inflammatory Load - metabolomics |
| Project Summary: | The intestinal barrier, and the gut-associated lymphoid tissue, are the interface of the host-microbiome/pathogens interactions and, its disruption, has been associated with a series of inflammatory, autoimmune, and degenerative diseases. Herein we performed the analysis of the pre-nodal mesenteric lymph at steady state and following disruption of the intestinal barrier to map the antigenic and pro-inflammatory load in both mice and human subjects with inflammatory bowel disease. The antigenic signature of gastrointestinal tissue inflammation was reflected in the mesenteric nodal dendritic-cells (DC)-MHCII-eluted immunopeptidome, in a tissue specific manner, when compared to the DC-eluted-MHCII-peptidome from cervical lymph nodes. Pro-inflammatory and microbiome-derived by-products, such as amino acids, deoxy sugars, component of the bacterial wall and other immunomodulators of the gut-brain axis, were found in the afferent lymph following damage to the gut epithelium. Our data points to the relevance of the lymphatic fluid to probe the tissue-specific antigenic and inflammatory load transported to the draining lymph node. |
| Institute: | University of Colorado Denver |
| Laboratory: | Lab of Angelo D'Alessandro in collaboration with lab of Laura Santambrogio (Weill Cornell) |
| Last Name: | Haines |
| First Name: | Julie |
| Address: | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| Email: | julie.haines@cuanschutz.edu |
| Phone: | 3037243339 |
Summary of all studies in project PR001770
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002828 | Role of the Afferent Lymph as an Immunological Conduit to Analyze Tissue Antigenic and Inflammatory Load - metabolomics | Mus musculus | University of Colorado Denver | MS | 2024-08-21 | 1 | 22 | Uploaded data (1.6G)* |
