Summary of project PR001820
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001820. The data can be accessed directly via it's Project DOI: 10.21228/M8G13Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001820 |
| Project DOI: | doi: 10.21228/M8G13Q |
| Project Title: | Metabolomics Discovery of Aryl Hydrocarbon Receptor Activating Metabolites from the Human Microbiota (Targeted) |
| Project Type: | Bacteria supernatant |
| Project Summary: | The aryl hydrocarbon receptor (AhR) is a transcription factor that regulates gene expression upon activation by small molecules. It plays a significant role in the innate immune recognition of bacteria and response to exogenous molecules in the human host. By stimulating host immune cells with microbiota metabolites, the AhR signaling enables microbiota-dependent induction, training, and function of the host immune system. AhR is a potential target for developing therapeutics to treat myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), cancer, and aging-related diseases. A variety of bioactive molecules can act as AhR agonists, including the metabolites and derivatives of indole and tryptophan. However, given the ligand-binding versatility of AhR, new methods are needed to discover novel AhR agonists. Herein, we report an analytical workflow for the deep discovery of AhR agonists from the secreted metabolome of bacteria. We also describe a method of targeted discovery of AhR-activating metabolites and report a correlation analysis of the AhR activity with the concentration of endogenous metabolites that identified significant common AhR activators shared by different strains of bacteria in the human microbiome. Principal component analysis of relative concentrations of indole compounds clusters different bacteria species, providing a possible means for evaluating the regulation of the bacteria indole pathway. |
| Institute: | University of Connecticut |
| Department: | Chemistry |
| Laboratory: | Yao Lab |
| Last Name: | Tian |
| First Name: | Huidi |
| Address: | 55 N. Eagleville Road, Unit 3060, Storrs CT 06269 |
| Email: | huidi.tian@uconn.edu |
| Phone: | 8606341143 |
| Funding Source: | NIH-NINDS and NIH-NIAID |
Summary of all studies in project PR001820
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002308 | Metabolomics profiling of full extracts and fractions of bacterial culture supernatants. | Species specified in the sample list | University of Connecticut | MS | 2025-10-05 | 1 | 234 | Uploaded data (1.6G)* |
