Summary of project PR001851
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001851. The data can be accessed directly via it's Project DOI: 10.21228/M8FT6G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001851 |
| Project DOI: | doi: 10.21228/M8FT6G |
| Project Title: | Leishmania mexicana Promotes Pain-reducing Metabolomic Reprogramming In Cutaneous Lesions |
| Project Type: | Untargeted MS |
| Project Summary: | Cutaneous leishmaniasis is characterized by extensive skin lesions, which are usually painless despite being associated with extensive inflammation. The molecular mechanisms responsible for this analgesia have not been identified. Through untargeted metabolomics, we found enriched anti-nociceptive metabolic pathways in L. mexicana-infected mice. Purines were elevated in infected macrophages and at the lesion site during chronic infection. These purines have anti-inflammatory and analgesic properties by acting through adenosine receptors, inhibiting TRPV1 channels, and promoting IL-10 production. We also found arachidonic acid metabolism enriched in the ear lesions compared to the non-infected controls. Arachidonic acid is a metabolite of anandamide (AEA) and 2-arachidonoylglycerol (2-AG). These endocannabinoids act on cannabinoid receptors 1 and 2 and TRPV1 channels to exert anti-inflammatory and analgesic effects. Our study provides evidence of metabolic pathways upregulated during L. mexicana infection that may mediate anti-nociceptive effects experienced by CL patients and identifies macrophages as a source of these metabolites. |
| Institute: | The Ohio State University |
| Department: | Pathology and Microbiology |
| Last Name: | Satoskar |
| First Name: | Abhay |
| Address: | Evans Hall, 520 King Avenue, Columbus, OH, 43201, USA |
| Email: | abhay.satoskar@osumc.edu |
| Phone: | (614) 293-0537 |
Summary of all studies in project PR001851
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002974 | Leishmania mexicana Promotes Pain-reducing Metabolomic Reprogramming In Cutaneous Lesions | Mus musculus | The Ohio State University | MS* | 2024-10-18 | 1 | 66 | Uploaded data (8.6M) |
