Summary of project PR001868
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001868. The data can be accessed directly via it's Project DOI: 10.21228/M8842H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001868 |
| Project DOI: | doi: 10.21228/M8842H |
| Project Title: | From clinical to benchside: Lacticaseibacillus and Faecalibacterium are positively associated with muscle health and alleviate age-related muscle disorder |
| Project Summary: | Sarcopenia is an age-related muscle disorder that increases the risk of adverse clinical outcomes, but its pathogenesis is unclear and treatments are limited. Increasing evidence shows that gut microbiota is potentially associated with sarcopenia. To investigate its role in sarcopenia, we (i) compared gut microbiota and metabolite composition between older persons with and without sarcopenia, (ii) performed fecal microbiota transplantation (FMT) from human donors to antibiotic-treated mice, and (iii) identified two specific probiotics for treatment of sarcopenia in aged mice. Metagenomic and metabolomic analyses showed that people with sarcopenia had different microbial composition and metabolites, and that fecal purine could accurately identify sarcopenia. After FMT, mice receiving microbes from people with sarcopenia displayed lower muscle mass and strength. Correlation analysis revealed Lacticaseibacillus rhamnosus (LR) and Faecalibacterium prausnitzii (FP) were positively related to muscle health in old people. Both probiotic LR, FP and their combination enhanced muscle mass, function, and fiber type proportion of aged mice. Transcriptomics showed that genes related to tricarboxylic acid cycle were enriched after treatment. Mitochondria density, muscle ATP content, NAD+/NADH, proteins related to mitochondrial dynamics and biogenesis were improved by both probiotics. In in-vitro studies, probiotic-conditional medium (PCM) containing FP supernatant or the combination of FP and LR supernatants enhanced proliferation of C2C12 myoblasts, whilst LR PCM alone did not. The mechanisms of LR may be related to colon health improvement. Results showed gut microbiota dysbiosis is one of pathogenic factors of sarcopenia, and muscle-related probiotics could alleviate age-related muscle disorders. Further clinical translation is warranted. |
| Institute: | The Chinese University of Hong Kong |
| Department: | ORT |
| Laboratory: | The Prince of Wales Hospital |
| Last Name: | Wong |
| First Name: | Pui Yan |
| Address: | 30-32 Ngan Shing Street , Shatin, New Territories |
| Email: | puiyanwong@cuhk.edu.hk |
| Phone: | (852)-35052756 |
Summary of all studies in project PR001868
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002998 | The role of gut microbiota in muscle mitochondria function, colon health, and sarcopenia: from clinical to bench | Lacticaseibacillus rhamnosus and Faecalibacterium prausnitzii | The Chinese University of Hong Kong | MS | 2024-12-30 | 1 | 12 | Uploaded data (97.6M)* |
| ST003002 | The role of gut microbiota in muscle mitochondria function, colon health, and sarcopenia: from clinical to bench (2) | Homo sapiens | The Chinese University of Hong Kong | MS | 2024-12-30 | 1 | 51 | Uploaded data (283.3M)* |
