Summary of project PR001878
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001878. The data can be accessed directly via it's Project DOI: 10.21228/M8ZQ63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001878 |
| Project DOI: | doi: 10.21228/M8ZQ63 |
| Project Title: | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) |
| Project Summary: | Florida manatees (Trichechus manatus latirostris) are protected as a threatened species, and data are lacking regarding their reproductive physiology. This study aimed to (1) quantify plasma steroid hormones in Florida manatees from two field sites, Crystal River and Indian River Lagoon, at different gestational stages and to (2) determine the relationship between plasma progesterone concentrations and lipid biochemistry in relation to pregnancy status. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis was used to measure plasma steroid hormones and lipids. Pregnant female manatees were morphometrically distinct from male and non-pregnant female manatees, characterized by larger body weight and maximal girth. Progesterone concentrations in manatees were also elevated during early gestation versus late gestation. Cholesterol, an important metabolic lipid and precursor for reproductive steroids, was not different between groups. Lipidomics quantified 949 lipids and plasma concentrations of a sphingolipid, ceramide non-hydroxy fatty acid-sphingosine and several glycerophospholipids, including lysophosphatidylcholine, phosphatidylethanolamines, plasmenyl-phosphatidylserines and monomethyl phosphatidylethanolamines, were associated with pregnancy status in the Florida manatee. This research contributes to improving knowledge of manatee reproductive physiology by providing data on plasma steroid hormones relative to reproductive status and by assessing how plasma lipids in healthy Florida manatees correspond to progesterone levels. This lipid panel has potential as a diagnostic approach to identify pregnant individuals in fresh and archived samples. These biochemical and morphometric indicators of reproductive status advance the understanding of manatee physiology. |
| Institute: | University of Florida |
| Department: | Physiological Sciences |
| Laboratory: | Dr. Martyniuk |
| Last Name: | Brammer-Robbins |
| First Name: | Elizabeth |
| Address: | 2187 Mowry Rd., Bldg 471 |
| Email: | e.brammerrobbins@ufl.edu |
| Phone: | 9104652899 |
| Funding Source: | National Science Foundation Graduate Research Fellowship Program under Grant No. (2019285699 to EB-R). |
Summary of all studies in project PR001878
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003017 | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) | Florida manatee (Trichechus manatus latirostris) | University of Florida | MS* | 2024-06-12 | 1 | 67 | Uploaded data (13.9G)* |
