Summary of project PR001952
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001952. The data can be accessed directly via it's Project DOI: 10.21228/M8DD95 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001952 |
| Project DOI: | doi: 10.21228/M8DD95 |
| Project Title: | Spatial analysis of murine GI reveals role of small intestinal bile acid metabolism in amoxicillin-induced dysbiosis |
| Project Summary: | Antibiotics cause collateral damage to resident microbes that is associated with various health risks. To-date, studies have largely focused on impacts of antibiotics on large intestinal and fecal microbiota. Here, we employ a GI-wide integrated multiomic approach to reveal that amoxicillin (AMX) treatment reduces overall bacterial abundance, bile salt hydrolase activity and unconjugated bile acids in the small intestine (SI). An accompanying loss of fatty acids and increase in acyl-carnitines in the large intestine corresponded with spatially-distinct expansions of proteobacteria. Parasutterella excrementihominis utilized fatty acid biosynthesis, becoming dominant in the SI while multiple Klebsiella species employed fatty acid oxidation during expansion in the large intestine. Depletion of bile acids and lipids may contribute to AMX-induced dysbiosis in the lower GI. To test this, we demonstrate that restoration of unconjugated bile acids can mitigate losses of commensals in the large intestine while also inhibiting the expansion of Proteobacteria during AMX treatment. |
| Institute: | Brown University |
| Department: | MMI |
| Laboratory: | MMI, Belenky Lab |
| Last Name: | Beekman |
| First Name: | Chapman |
| Address: | BMC 613, 171 Meeting Street, Providence RI 02912 |
| Email: | Chapman_Beekman@brown.edu |
| Phone: | 4012071832 |
Summary of all studies in project PR001952
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003140 | Untargeted metabolomic analysis of lumenal contents from AMX-treated and untreated mice | Mus musculus | Brown University | MS* | 2024-08-05 | 1 | 84 | Uploaded data (613.1K) |
| ST003141 | untargeted metabolomic analysis of whole blood from AMX-treated and untreated mice | Mus musculus | Brown University | MS* | 2024-08-05 | 1 | 12 | Uploaded data (85.7K) |
| ST003277 | LC-MS/MS spatial analysis of mouse GI | Mus musculus | Brown University | MS | 2024-08-05 | 1 | 84 | Not available |
