Summary of project PR001957
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001957. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ80 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001957 |
| Project DOI: | doi: 10.21228/M8RQ80 |
| Project Title: | Exploring The Impact of Two Novel DNA Minor Groove Binders on HCT-116 Cells: A Comprehensive Multi-Omics Analysis Using Mass Spectrometry |
| Project Summary: | Colorectal cancer (CRC) poses a significant global health challenge, necessitating innovative therapeutic approaches. Despite advancements, current treatments encounter obstacles such as chemotherapy resistance and adverse effects due to non-selective targeting. DNA Minor Groove Binders (MGBs) present promising alternatives, targeting DNA structure without causing permanent damage. In this study, two novel MGB compounds were synthesized, MGB30 and MGB32, resembling distamycin, a natural DNA-binding agent. These compounds bind reversibly to the DNA minor groove, influencing DNA structure and inhibiting cancer growth-related enzymes. Our study aims to explore the unique effects of MGB30 and MGB32 on the metabolomic profiles of treated HCT-116 cells using TIMS-QTOF-UHPLC-MS. Objectives include comprehensive analysis, comparison of effects, identification of altered pathways, and insights into MGB compound mechanisms. Additionally, we established four biological replicates for each treatment condition. Advanced statistical analyses, including the two-tailed independent Student's t-test and one-way analysis of variance (ANOVA), were utilized to minimize false discoveries. Our analysis generated a comprehensive dataset from 12 samples, identifying 75 distinct metabolites. The significance of this study lies in elucidating the molecular mechanisms of action of MGB30 and MGB32, crucial for their development as CRC drug candidates. |
| Institute: | Sharjah Institute for Medical Research |
| Last Name: | Facility |
| First Name: | Core |
| Address: | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah, Sharjah, UAE, Sharjah, 000, United Arab Emirates |
| Email: | tims-tof@sharjah.ac.ae |
| Phone: | +971 6 5057656 |
Summary of all studies in project PR001957
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003146 | Exploring The Impact of Two Novel DNA Minor Groove Binders on HCT-116 Cells: A Comprehensive Multi-Omics Analysis Using Mass Spectrometry | Homo sapiens | Sharjah Institute for Medical Research | MS | 2024-07-29 | 1 | 24 | Uploaded data (9.9G)* |
