Summary of project PR001986
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001986. The data can be accessed directly via it's Project DOI: 10.21228/M81142 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001986 |
| Project DOI: | doi: 10.21228/M81142 |
| Project Title: | A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate the inositol hexaphosphate accumulation |
| Project Type: | research |
| Project Summary: | Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a previously uncharacterized regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding new light on the mechanisms of InsP biosynthesis in eukaryotes. |
| Institute: | zhejiang University |
| Last Name: | XU |
| First Name: | LILIN |
| Address: | 866 yuhangtang road, hangzhou, zhejiang, 310027, China |
| Email: | 1164702127@qq.com |
| Phone: | 18667919279 |
Summary of all studies in project PR001986
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003190 | A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate the inositol hexaphosphate accumulation | Arabidopsis thaliana | Zhejiang University | MS | 2024-05-08 | 1 | 24 | Uploaded data (1.7M)* |
