Summary of project PR002013
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002013 |
| Project DOI: | doi: 10.21228/M8DJ7S |
| Project Title: | Glutathione synthesis in the mouse liver supports lipid abundance through NRF2 repression. |
| Project Summary: | Cells rely on antioxidants to survive. The most abundant antioxidant is glutathione (GSH). The synthesis of GSH is non-redundantly controlled by the glutamate-cysteine ligase catalytic subunit (GCLC). GSH imbalance is implicated in many diseases, but the requirement for GSH in adult tissues is unclear. To interrogate this, we have developed a series of in vivo models to induce Gclc deletion in adult animals. We find that GSH is essential to lipid abundance in vivo. GSH levels are highest in liver tissue, which is also a hub for lipid production. While the loss of GSH does not cause liver failure, it decreases lipogenic enzyme expression, circulating triglyceride levels, and fat stores. Mechanistically, we find that GSH promotes lipid abundance by repressing NRF2, a transcription factor induced by oxidative stress. These studies identify GSH as a fulcrum in the liver's balance of redox buffering and triglyceride production. |
| Institute: | University of Rochester Medical Center |
| Last Name: | Harris |
| First Name: | Isaac |
| Address: | 601 Elmwood Ave, Rochester, New York, 14642-0001, USA |
| Email: | isaac_harris@urmc.rochester.edu |
| Phone: | 8572348624 |
Summary of all studies in project PR002013
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003238 | Lipidomic analysis of serum from Gclc WT and whole-body Gclc KO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-07 | 1 | 8 | Uploaded data (2.7G)* |
| ST003239 | Lipidomic analysis of adipose from Gclc WT and whole-body Gclc KO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-10 | 1 | 7 | Uploaded data (2.4G)* |
| ST003240 | Lipidomic analysis of liver from Gclc WT and whole-body Gclc KO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-07 | 1 | 8 | Uploaded data (2.6G)* |
| ST003241 | Lipidomic analysis of lung from Gclc WT and whole-body Gclc KO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-07 | 1 | 8 | Uploaded data (2.7G)* |
| ST003242 | Lipidomic analysis of kidney from Gclc WT and whole-body Gclc KO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-07 | 1 | 8 | Uploaded data (2.4G)* |
| ST003243 | Lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. | Mus musculus | University of Rochester Medical Center | MS | 2024-06-07 | 1 | 18 | Uploaded data (2.6G)* |
