Summary of project PR002018
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002018. The data can be accessed directly via it's Project DOI: 10.21228/M8RV57 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002018 |
| Project DOI: | doi: 10.21228/M8RV57 |
| Project Title: | Temperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B |
| Project Type: | Publication |
| Project Summary: | Cell membranes mediate interactions between life and its environment, with lipids determining their properties. Understanding how cells adjust their lipidomes to tune membrane properties is crucial yet poorly defined due to the complexity of most organisms. We used quantitative shotgun lipidomics to study temperature adaptation in the simple organism Mycoplasma mycoides and the minimal cell, JCVI-syn3B. We show that lipid abundances follow a universal logarithmic distribution across eukaryotes and bacteria, with comparable degrees of lipid remodeling for adaptation regardless of lipidomic or organismal complexity. Lipid features analysis demonstrates head group-specific acyl chain remodeling as characteristic of lipidome adaptation; its deficiency in Syn3B is associated with impaired homeoviscous adaptation. Temporal analysis reveals a two-stage cold adaptation process: swift cholesterol and cardiolipin shifts followed by gradual acyl chain modifications. This work provides an in-depth analysis of lipidome adaptation in minimal cells, laying a foundation to probe the design principles of living membranes. |
| Institute: | TU Dresden |
| Last Name: | Saenz |
| First Name: | James |
| Address: | Tatzberg 41, Dresden, Saxony, 01307, Germany |
| Email: | jpsaenz81@gmail.com |
| Phone: | (+49) 351 463 43066 |
Summary of all studies in project PR002018
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003251 | Temperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B | Mycoplasma mycoides, Minimal cell JCVI-syn3B | TU Dresden | MS | 2024-06-12 | 1 | 34 | Uploaded data (1.8M) |
