Summary of project PR002026
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002026. The data can be accessed directly via it's Project DOI: 10.21228/M8QV5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002026 |
| Project DOI: | doi: 10.21228/M8QV5X |
| Project Title: | SLC25A48 controls mitochondrial choline import and metabolism |
| Project Summary: | Choline is an essential nutrient for cellular metabolism, including the biosynthesis of phospholipids, neurotransmitters, and one-carbon metabolism. A critical step in choline catabolism is the mitochondrial import and synthesis of choline-derived methyl donors, such as betaine. However, the underlying mechanisms and the biological significance of mitochondrial choline metabolism remain poorly understood. Here, we report that a previously uncharacterized mitochondrial inner-membrane protein, SLC25A48, controls mitochondrial choline transport and catabolism in vivo. We show that SLC25A48 is highly expressed in brown adipose tissue and is required for whole-body cold tolerance, thermogenesis, mitochondrial respiration, and mitochondrial membrane integrity. Choline uptake into the mitochondria via SLC25A48 facilitates the synthesis of betaine and purine nucleotides, whereas loss of SLC25A48 resulted in increased production of reactive oxygen species and imbalanced mitochondrial lipids. Notably, human cells carrying a single nucleotide polymorphism on the SLC25A48 gene and cancer cells lacking SLC25A48 exhibited elevated oxidative stress and impaired cell proliferation. Together, the present study identified SLC25A48 as a mitochondrial carrier that mediates choline catabolism and plays a critical role in mitochondrial function and cell survival. |
| Institute: | Beth Israel Deaconess Medical Center |
| Last Name: | Verkerke |
| First Name: | Anthony |
| Address: | 330 Brookline Avenue, Boston, Massachusetts, 02215, USA |
| Email: | averkerk@bidmc.harvard.edu |
| Phone: | 7159231910 |
Summary of all studies in project PR002026
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003264 | SLC25A48 controls mitochondrial choline import and metabolism | Mus musculus | Harvard Medical School | MS | 2024-06-20 | 1 | 16 | Uploaded data (2.3G)* |
