Summary of project PR002043
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002043. The data can be accessed directly via it's Project DOI: 10.21228/M8J52B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002043 |
| Project DOI: | doi: 10.21228/M8J52B |
| Project Title: | Imaging mass spectrometry evaluation of wound healing in patients with atopic dermatitis with and without STAT3 dominant negative mutations. |
| Project Summary: | Frequent injury due to environmental challenges requires constant restoration of the skin barrier. Specific defects in ceramide lipid metabolism have been associated with inflammatory skin diseases such as atopic dermatitis (AD). The goal of this study was to characterize the lipid landscape and biosynthetic pathways activated during epidermal repair using MALDI imaging mass spectrometry (IMS). A keratinocyte culture model of wound healing confirmed that glycerophospholipids and glycosphingolipids are impacted during cell proliferation and that sialic acid supplementation can decrease wound closure time. In skin biopsies of healing tissue taken from healthy volunteers, specific phospholipids were upregulated, including phosphatidylcholine and sphingolipids, which play critical roles in membrane repair and cell signaling. Comparing controls to patients with AD, we again identified defects in ceramide-related lipid metabolism with specific defects in lysophosphatidylcholine (Lyso PC) 18:1. Topical application of LysoPC 18:1 provided modest clinical improvement in a mouse model of AD. We then applied this workflow to samples taken from patients with eczematous phenotypes due to STAT3 dominant negative hyper IgE syndrome and identified metabolic disruption of thiamine and phosphatidylinositol metabolism. Overall, the results indicate MALDI imaging can be used to characterize changes in lipid metabolism during wound healing and may indicate an underappreciated role of LysoPC 18:1 during tissue repair. |
| Institute: | National Institutes of Health |
| Department: | National Institute of Allergy and Infectious Disease |
| Laboratory: | Epithelial Therapeutics Unit, |
| Last Name: | Ratley |
| First Name: | Grace |
| Address: | 9000 Rockville Pike, Bethesda, MD, 20814, USA |
| Email: | grace.ratley@nih.gov |
| Phone: | 8507371782 |
Summary of all studies in project PR002043
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003291 | Atopic dermatitis and STAT3 double null mutant skin biopsies during healing. | Homo sapiens | National Institutes of Health | MS | 2024-07-25 | 1 | 114 | Uploaded data (82.9M)* |
