Summary of project PR002065
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002065. The data can be accessed directly via it's Project DOI: 10.21228/M8PR7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002065 |
| Project DOI: | doi: 10.21228/M8PR7B |
| Project Title: | Changes in phospholipid fatty acid composition under cytotoxic stress facilitate peroxidation |
| Project Summary: | Programs leading to cell death, such as apoptosis, necroptosis, and ferroptosis, involve an oxidative component linked to lipid metabolism that influences membrane homeostasis. Emerging evidence suggests inter-program cross-talk, emphasizing the need for overarching regulatory mechanisms. We show that under specific cytotoxic stress conditions, exogenous or released polyunsaturated fatty acids (PUFAs) are channeled into overall depleting phospholipids that become vulnerable to peroxidation in the presence of associated redox stress. In fibroblasts, this reprogramming results from reduced growth factor receptor tyrosine kinase (RTK) and phosphatidylinositol-3-kinase (PI3K)/Akt signaling, which reduces de novo fatty acid biosynthesis by mechanisms that differ depending on the specific cytotoxic stressors. We conclude that alterations in PUFA metabolism during cytotoxic stress render cells prone to oxidative modifications in phospholipids. |
| Institute: | University of Innsbruck |
| Department: | Michael Popp Institute |
| Last Name: | Koeberle |
| First Name: | Andreas |
| Address: | Mitterweg 24, Innsbruck, Tyrol, 6020, Austria |
| Email: | andreas.koeberle@uibk.ac.at |
| Phone: | +43 512 507 57903 |
| Funding Source: | Austrian Science Fund (FWF) (P 36299). German Research Council (GRK 1715 and KO 4589/4-1), the Phospholipid Research Center Heidelberg (AKO-2015-037/1-1, AKO-2019-070/2-1, AKO-2O22-100/2-2), the University of Jena (DRM/2013-05 and 2.7-05). |
| Contributors: | André Gollowitzer, Helmut Pein, Konstantin Loeser, Maria Thuermer, and Andreas Koeberle |
Summary of all studies in project PR002065
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003318 | Kinetic changes in the phospholipid composition of fibroblasts induced by cytotoxic stress | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 684 | Uploaded data (24.5M)* |
| ST003319 | Changes in the phosphatidylcholine fatty acid composition in subcellular fractions of fibroblasts induced by valinomycin | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 12 | Uploaded data (658.6K)* |
| ST003320 | Time-dependent changes in the phosphatidylcholine fatty acid composition of fibroblasts treated with acetyl-CoA carboxylase inhibitors | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 61 | Uploaded data (4.4M)* |
| ST003321 | Effect of ACC inhibition on the proportion of PUFAs in phosphatidylcholines in stressed fibroblasts | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 30 | Uploaded data (1.2M)* |
| ST003336 | Differentiation between cytotoxic stress-induced PUFA biosynthesis and PUFA incorporation into phospholipids by metabolic flux studies in fibroblasts | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 30 | Uploaded data (1.6M)* |
| ST003337 | Analysis of oxidized arachidonoyl-phosphatidylethanolamine in valinomycin-treated fibroblasts | Mus musculus | University of Innsbruck | MS | 2025-01-06 | 1 | 7 | Uploaded data (828.7K)* |
