Summary of project PR002073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002073. The data can be accessed directly via it's Project DOI: 10.21228/M8NR71 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002073 |
| Project DOI: | doi: 10.21228/M8NR71 |
| Project Title: | Impact of partial body shielding from very high dose rates on untargeted metabolomics in biodosimetry |
| Project Summary: | A realistic exposure to ionizing radiation (IR) from an improvised nuclear device will likely include individuals that are partially shielded from the initial blast delivered at a very high-dose rate (VHDR). As different tissues have varying levels of radiosensitivity, e.g. hematopoietic vs. gastrointestinal tissues, the effects of shielding on radiation biomarkers needs to be addressed. Here, we explore how biofluid (urine and serum) metabolite signatures from male and female C57BL/6 mice exposed to VHDR (5 – 10 Gy/sec) total body irradiation (TBI, 0, 4, and 8 Gy) compare to individuals exposed to partial body irradiation (PBI) (lower body irradiated [LBI] or upper body irradiated [UBI] at an 8 Gy dose) using a data-independent acquisition untargeted metabolomics approach. Although sex differences were observed in the spatial groupings of urine signatures from TBI and PBI mice, a metabolite signature (N6,N6,N6-trimethyllysine, carnitine, propionylcarnitine, hexosamine-valine-isoleucine, taurine, and creatine) previously developed from variable dose rate experiments was able to identify individuals with high sensitivity and specificity irrespective of radiation shielding. A panel of serum metabolites composed from previous untargeted studies on nonhuman primates had excellent performance for separating irradiated cohorts; however, a multi-omic approach to complement the metabolome could increase dose estimation confidence intervals. Overall, these results support the inclusion of small molecule markers in biodosimetry assays without substantial interference from upper or lower body shielding. |
| Institute: | Georgetown University |
| Last Name: | Pannkuk |
| First Name: | Evan |
| Address: | 3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, 20057, USA |
| Email: | elp44@georgetown.edu |
| Phone: | 2026875650 |
Summary of all studies in project PR002073
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003334 | Impact of partial body shielding from very high dose rates on untargeted metabolomics in biodosimetry | Mus musculus | Georgetown University | MS* | 2024-12-02 | 1 | 74 | Uploaded data (1.8M) |
