Summary of project PR002075
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002075. The data can be accessed directly via it's Project DOI: 10.21228/M8D824 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002075 |
| Project DOI: | doi: 10.21228/M8D824 |
| Project Title: | The effect of rewarming ischemia on tissue transcriptome and metabolome signatures: a clinical observational study in lung transplantation |
| Project Type: | Clinical observational study in lung transplantation |
| Project Summary: | BACKGROUND: In lung transplantation (LuTx), various ischemic phases exist, yet the rewarming ischemia time (RIT) during implantation has often been overlooked. During RIT, lungs are deflated and exposed to the body temperature in the recipient's chest cavity. Our prior clinical findings demonstrated that prolonged RIT increases the risk of primary graft dysfunction. However, the molecular mechanisms of rewarming ischemic injury in this context remain unexplored. We aimed to characterize the rewarming ischemia phase during LuTx by measuring organ temperature and comparing transcriptome and metabolome profiles in tissue obtained at the end versus the start of implantation. METHODS: In a clinical observational study, 34 double-LuTx with ice preservation were analyzed. Lung core and surface temperature (n=65 and 55 lungs) was measured during implantation. Biopsies (n=59 lungs) were wedged from right middle lobe and left lingula at start and end of implantation. Tissue transcriptomic and metabolomic profiling were performed. RESULTS: Temperature increased rapidly during implantation, reaching core/surface temperatures of 21.5°C/25.4°C within 30min. Transcriptomics showed increased pro-inflammatory signaling and oxidative stress at the end of implantation. Upregulation of NLRP3 and NFKB1 correlated with RIT. Metabolomics indicated elevated levels of amino acids, hypoxanthine, uric acid, cysteineglutathione disulfide alongside decreased levels of glucose and carnitines. Arginine, tyrosine, and 1-carboxyethylleucine showed correlation with incremental RIT. CONCLUSIONS: The final rewarming ischemia phase in LuTx involves rapid organ rewarming, accompanied by transcriptomic and metabolomic changes indicating pro-inflammatory signaling and disturbed cell metabolism. Limiting implantation time and lung cooling represent potential interventions to alleviate rewarming ischemic injury. |
| Institute: | KU Leuven |
| Laboratory: | Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE) |
| Last Name: | Ceulemans |
| First Name: | Laurens |
| Address: | Herestraat 49, Leuven, Vlaams-Brabant, 3000, Belgium |
| Email: | laurens.ceulemans@uzleuven.be |
| Phone: | +32 16 34 34 25 |
Summary of all studies in project PR002075
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003338 | GRATEFUL (Graft Temperature Evaluation during Lung transplantation) - metabolomics | Homo sapiens | KU Leuven | MS* | 2024-08-12 | 1 | 1416 | Uploaded data (60.9G)* |
