Summary of project PR002085
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002085. The data can be accessed directly via it's Project DOI: 10.21228/M83R6P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002085 |
| Project DOI: | doi: 10.21228/M83R6P |
| Project Title: | Altered sphingolipid biosynthetic flux and lipoprotein trafficking contribute to trans fat-induced atherosclerosis |
| Project Summary: | The goal of the project is to determine the role of sphingolipid metabolism in atherosclerosis induced by dietary trans fat. We analyzed lipid metabolites in Huh7 cells following various fatty acid treatments, with specific focus on cis and trans unsaturated fatty acids. Additionally, we analyzed lipid metabolites in plasma and liver of Ldlr-/- mice fed high-fat diets enriched in cis or trans fatty acids in the presence or absence of myriocin, a pharmacological inhibitor of Serine palmitoyltransferase (SPT), the initial rate-limiting enzyme of sphingolipid biosynthesis. |
| Institute: | Salk Institute for Biological Studies |
| Last Name: | Gengatharan |
| First Name: | Jivani |
| Address: | 10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA |
| Email: | jivani14@gmail.com |
| Phone: | (858) 453-4100 |
Summary of all studies in project PR002085
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003351 | Incorporation of various fatty acids in long-chain base of sphingolipids in Huh7 cells | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 24 | Uploaded data (302.7M)* |
| ST003353 | Incorporation of oleate-d9 and elaidate-d17 in long-chain base of sphingolipids in Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 12 | Uploaded data (120.7M)* |
| ST003354 | Incorporation of co-treated oleate-d9 and elaidate-d17 in long-chain base of sphingolipids in Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 3 | Uploaded data (32.4M)* |
| ST003355 | Incorporation of two types of cis and trans fatty acids in long-chain base of sphingolipids in Huh7 cells | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 15 | Uploaded data (164.2M)* |
| ST003357 | Incorporation of oleate-d9 and elaidate-d17 in sphingolipids in Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 12 | Uploaded data (122.3M)* |
| ST003358 | Secretion of sphingomyelin from Huh7 cells treated with various fatty acids including of oleate-d9 and elaidate-d17. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 24 | Uploaded data (218.9M)* |
| ST003359 | Secretion of phosphatidylcholine from Huh7 cells treated with oleate-d9 or elaidate-d17 | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-09 | 1 | 12 | Uploaded data (55.6M)* |
| ST003360 | Secretion of sphingomyelin from Huh7 cells treated with oleate-d9 or elaidate-d17 while modulating SPT flux. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 15 | Uploaded data (155.1M)* |
| ST003369 | Incorporation of oleate-d9 and elaidate-d17 in Lac-Cer and SM in Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 6 | Uploaded data (66.7M)* |
| ST003371 | Incorporation of oleate-d9 and elaidate-d17 into membrane lipids of Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 6 | Uploaded data (45.2M)* |
| ST003372 | Incorporation of oleate-d9 and elaidate-d17 into neutral lipids of Huh7 cells. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 6 | Uploaded data (33.6M)* |
| ST003373 | Incorporation of oleate-d9 and elaidate-d17 in sphingolipids in Huh7 cells while modulating SPT flux. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 12 | Uploaded data (106.5M)* |
| ST003374 | Incorporation of oleate-d9 and elaidate-d17 into complex sphingolipids in Huh7 cells while modulating SPT flux. | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 12 | Uploaded data (123.1M)* |
| ST003377 | Sphingolipid secretory flux from Huh7 SPTLC3 KO cells | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 14 | Uploaded data (510.2M)* |
| ST003379 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on hepatic lipidome in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 40 | Uploaded data (1.1G)* |
| ST003381 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on newly synthesized sphingolipids from D2O in circulation in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 23 | Uploaded data (866.2M)* |
| ST003382 | Sphingolipid biosynthetic flux in Huh7 SPTLC3 KO cells | Homo sapiens | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 11 | Uploaded data (365.6M)* |
| ST003383 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on the plasma lipoprotein profile of sphingolipids in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 264 | Uploaded data (2.6G)* |
| ST003384 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on plasma lipidome in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 40 | Uploaded data (1.2G)* |
| ST003391 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on plasma sphingolipids in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 39 | Uploaded data (370.4M)* |
| ST003392 | Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on hepatic sphingolipids in Ldlr-/- mice | Mus musculus | Salk Institute for Biological Studies | MS | 2024-08-12 | 1 | 38 | Uploaded data (350.1M)* |
