Summary of project PR002102
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002102. The data can be accessed directly via it's Project DOI: 10.21228/M8X24G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002102 |
| Project DOI: | doi: 10.21228/M8X24G |
| Project Title: | Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction |
| Project Summary: | The gut microbiota influences physiological functions of the host, ranging from the maintenance of local gut homeostasis to systemic immunity and metabolism. Secreted phospholipase A2 group X (sPLA2-X) is abundantly expressed in colonic epithelial cells but is barely detectable in metabolic and immune tissues. Despite this distribution, sPLA2-X-deficient (Pla2g10-/-) mice displayed variable obesity-related phenotypes that were abrogated after treatment with antibiotics or cohousing with Pla2g10+/+ mice, suggesting the involvement of gut microbiota. Under housing conditions where Pla2g10-/- mice showed aggravation of diet-induced obesity and insulin resistance, they displayed increased colonic inflammation and epithelial damage, reduced production of polyunsaturated fatty acids (PUFAs) and lysophospholipids, decreased abundance of several Clostridium species, and reduced levels of short-chain fatty acids (SCFAs). These obesity-related phenotypes in Pla2g10-/- mice were reversed by dietary supplementation with omega-3 PUFAs or SCFAs. Thus, colonic sPLA2-X orchestrates omega-3 PUFA-SCFA interplay via modulation of gut microbiota, thereby secondarily affecting systemic metabolism. |
| Institute: | University of Tokyo |
| Last Name: | Sato |
| First Name: | Hiroyasu |
| Address: | Tokyo 113-8655, Japan. |
| Email: | sato-hr@m.u-tokyoac.jp |
| Phone: | 81-3-5841-1433 |
Summary of all studies in project PR002102
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003393 | Free fatty acid and lysophospholipids analysis of colon in Pla2g10 knockout mice. | Mus musculus | University of Tokyo | MS | 2024-08-30 | 1 | 83 | Uploaded data (4.2M)* |
| ST003394 | Fatty acid oxygenated metabolites analysis of colon in Pla2g10 knockout mice. | Mus musculus | University of Tokyo | MS | 2024-08-30 | 1 | 31 | Uploaded data (3M)* |
| ST003395 | Short chain fatty acids analysis of Feces and Plasma in Pla2g10 knockout mice. | Mus musculus | University of Tokyo | MS | 2024-08-30 | 1 | 110 | Uploaded data (2.5M)* |
