Summary of project PR002109
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002109. The data can be accessed directly via it's Project DOI: 10.21228/M80V5D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002109 |
| Project DOI: | doi: 10.21228/M80V5D |
| Project Title: | Deficiency in glutathione peroxidase 4 (GPX4) results in abnormal lens development and newborn cataract |
| Project Type: | Lipidocmics |
| Project Summary: | The human lens consists of a monolayer of lens epithelial cells and extensively elongated fibers that are tightly aligned but separated by the plasma membrane. The integrity of the lens plasma membrane is essential for maintaining lens cellular structure, homeostasis, and transparency. Glutathione peroxidase 4 (GPX4), a selenoenzyme, plays a critical role in protecting against lipid peroxidation. This study aims to elucidate the role of GPX4 in maintaining lens plasma membrane stability during lens development, utilizing in vitro, ex vivo, and in vivo systems. By employing a lipidomics approach, we aim to understand the phospholipid profile of the lens plasma membrane and its alterations following the deletion of GPX4, a key lipid peroxidation detoxification enzyme. Our findings reveal that the deletion of lens-specific GPX4 results in a significant loss of unsaturated phospholipids and an increase in oxidized phospholipids. Consequently, lenses deficient in GPX4 exhibit massive disruption of lens fiber cell structure, significant loss of lens epithelial cells via ferroptosis, and the formation of congenital cataracts. Our study underscores the crucial role of GPX4 in lens development and transparency and offers a potential intervention strategy to prevent lens developmental defects by inhibiting lipid peroxidation. |
| Institute: | Augusta University |
| Last Name: | Fan |
| First Name: | Xingjun |
| Address: | 1460 Laney Walker Blvd, Augusta, GA 30912 |
| Email: | xfan@augusta.edu |
| Phone: | 7067212019 |
| Funding Source: | NEI |
Summary of all studies in project PR002109
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003407 | Deficiency in glutathione peroxidase 4 (GPX4) results in abnormal lens development and newborn cataract | Mus musculus | Augusta University | MS | 2024-11-01 | 1 | 24 | Uploaded data (25.2M)* |
