Summary of project PR002115
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002115. The data can be accessed directly via it's Project DOI: 10.21228/M87C0V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002115 |
| Project DOI: | doi: 10.21228/M87C0V |
| Project Title: | TRAM–LAG1–CLN8 family proteins are acyltransferases regulating phospholipid composition |
| Project Summary: | The diversity of cellular phospholipids, crucial for membrane homeostasis and function, arises from enzymatic remodeling of their fatty acyl chains. In this work, we reveal that poorly understood TRAM–LAG1–CLN8 domain-containing (TLCD) proteins are phospholipid remodeling enzymes. We demonstrate that TLCD1 is an evolutionarily conserved lysophosphatidylethanolamine acyltransferase, which regulates cellular phospholipid composition and generates novel fatty acid and thiamine (vitamin B1) esters as its secondary products. Furthermore, we establish that human TLCD protein CLN8, mutations in which cause fatal neurodegenerative Batten disease, is a lysophosphatidylglycerol acyltransferase. We show that CLN8 catalyzes the essential step in the biosynthesis of bis(monoacylglycero)phosphate, a phospholipid critical for lysosome function. Our study unveils a new family of acyltransferases integral to cellular membrane phospholipid homeostasis and human disease. |
| Institute: | University of Cambridge |
| Last Name: | Petkevicius |
| First Name: | Kasparas |
| Address: | The Keith Peters Building, Cambridge, Cambridgeshire, CB2 0XY, United Kingdom |
| Email: | kp416@mrc-mbu.cam.ac.uk |
| Phone: | +447500233355 |
| Publications: | TRAM–LAG1–CLN8 family proteins are acyltransferases regulating phospholipid composition |
Summary of all studies in project PR002115
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003418 | Analysis of lipid composition of TLCD1 KO cultured cells | Homo sapiens | University of Cambridge | MS | 2024-11-11 | 1 | 31 | Uploaded data (2.2G)* |
| ST003419 | Analysis of lipid composition of the mitochondria isolated from TLCD1 KO cell models | Homo sapiens | University of Cambridge | MS | 2024-11-11 | 1 | 37 | Uploaded data (2.7G)* |
| ST003420 | Analysis of lipid composition of control, YPR114w and YJR116w yeast mutants grown under exponential phase | Saccharomyces cerevisiae | University of Cambridge | MS | 2024-11-11 | 1 | 10 | Uploaded data (766.2M)* |
| ST003424 | Analysis of lipid composition of HeLa and U2OS CLN8 KO cultured cells | Homo sapiens | University of Cambridge | MS | 2024-11-12 | 1 | 25 | Uploaded data (1.7G)* |
