Summary of project PR002118
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002118. The data can be accessed directly via it's Project DOI: 10.21228/M8V53H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002118 |
| Project DOI: | doi: 10.21228/M8V53H |
| Project Title: | Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in response to maternal obesity |
| Project Summary: | Maternal obesity puts the offspring at high risk of developing obesity and cardio-metabolic diseases in adulthood. Here, using a mouse model of maternal high-fat diet-induced obesity, we show that whole body fat content of the Off-HFD increases significantly from very early age when compared to the Off-RD. We have previously shown significant metabolic and immune perturbations in the bone marrow of newly weaned offspring of obese mothers. Therefore, we hypothesized that lipid metabolism is altered in the bone marrow Off-HFD in newly weaned offspring of obese mothers when compared to the Off-RD. To test this hypothesis, we investigated the lipidomic profile of bone marrow cells collected from three-week-old offspring of regular and high fat diet-fed mothers. Diacylgycerols (DAGs), triacylglycerols (TAGs), sphingolipids and phospholipids, including plasmalogen, and lysophospholipids were remarkably different between the groups, independent of fetal sex. Levels of cholesteryl esters were significantly decreased in offspring of obese mothers, suggesting reduced delivery of cholesterol to bone marrow cells. This was accompanied by age-dependent progression of mitochondrial dysfunction in bone marrow cells. We subsequently isolated CD11b+ myeloid cells from three-week-old mice and conducted metabolomics, lipidomics, and transcriptomics analyses. The lipidomic profiles of these bone marrow myeloid cells were largely similar to that seen in bone marrow cells and included increases in DAGs and phospholipids alongside decreased TAGs, except for long-chain TAGs, which were significantly increased. Our data also revealed significant sex-dependent changes in amino acids and metabolites related to energy metabolism. Transcriptomic analysis revealed altered expression of genes related to major immune pathways including macrophage alternative activation, B-cell receptor signaling, TGF signaling, and communication between the innate and adaptive immune systems. All told, this study revealed lipidomic, metabolomic, and gene expression abnormalities in bone marrow cells broadly, and in bone marrow myeloid cells particularly, in the newly-weaned offspring of obese mothers, which might at least partially explain the progression of metabolic and cardiovascular diseases in their adulthood. |
| Institute: | Oregon Health and Science University |
| Department: | Knight Cardiovascular Institute |
| Laboratory: | Maloyan Laboratory |
| Last Name: | Maloyan |
| First Name: | Alina |
| Address: | 3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239-3098, USA |
| Email: | maloyan@ohsu.edu |
| Phone: | 513-238-3783 |
Summary of all studies in project PR002118
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003426 | Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in response to maternal obesity | Mus musculus | Oregon Health and Science University | MS | 2025-08-19 | 1 | 24 | Uploaded data (226.3M)* |
