Summary of project PR002132
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002132. The data can be accessed directly via it's Project DOI: 10.21228/M81N7D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002132 |
| Project DOI: | doi: 10.21228/M81N7D |
| Project Title: | 1H NMR metabolomics applied to assess the direct and transgenerational effects of simvastatin on the metabolism of the amphipod Gammarus locusta |
| Project Type: | 1H NMR metabolomics to study the metabolic effects of direct exposure to simvastatin and transgenerational effects on the polar metabolome of amphipod Gammarus locusta |
| Project Summary: | Pharmaceutical compounds (PhACs) and their metabolites are considered contaminants of emerging concern (CECs) to public and environmental health, due to their high stability, bioactivity, and persistence in conventional wastewater treatment plants. Aquatic organisms can be chronically exposed to PhACs during critical periods of their life or even through multi-generations. This may result in severe physiological/metabolic/endocrine disturbances, not only through direct exposure but also through inter- and transgenerational inheritance (in the absence of the insult). Special attention is given to simvastatin, one of the PhACs most prescribed to humans for the primary treatment of hypercholesterolemia, known to affect endocrine functions and disrupt reproduction, development, neuronal processes and/or other important physiological responses; not only in exposed individuals, but also in subsequent non-exposed generations. Previous studies observed that direct exposure and transgenerational exposure to simvastatin has been found to impact severely on crustaceans’ reproduction, growth and development, although the underlying mechanism remains partially unclear. NMR metabolomic approaches may provide crucial complementary mechanistic information about the cascade of metabolic events occurring with exposure. In this study a comprehensive untargetd 1H NMR metabolomics study was applied to measure the metabolic effects of direct exposure to environmentally relevant concentrations of simvastatin (F0) and transgenerational exposure (F3, where only the F0 generation was exposed) on the keystone marine amphipod species Gammarus locusta. Furthermore, NMR is here employed for the first time to address G. locusta metabolic behavior. The obtained data added important knowledge, paving the way to an improved understanding of the metabolic events cascade associated with simvastatin exposure. |
| Institute: | University of Aveiro |
| Department: | CICECO – Aveiro Institute of Materials, Department of Chemistry |
| Laboratory: | Metabolomics Group |
| Last Name: | Rodrigues |
| First Name: | Joao A. |
| Address: | University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal |
| Email: | joao.rodrigues@ua.pt |
| Phone: | 00351234370707 |
| Funding Source: | This work was developed within TRANSEPIC − Exploring Transgenerational Epigenetic Inheritance: New Methods and Strategies to Improve Environmental Hazard and Risk Assessment of Key Contaminants of Emerging Concern (CECs) [Reference: 2022.02922.PTDC, doi: 10.54499/2022.02922.PTDC], financed by the Portuguese Foundation for Science and Technology (FCT). This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 (doi: 10.54499/UIDB/50011/2020), UIDB/50011/2020, UIDP/50011/2020 (doi: 10.54499/UIDP/50011/2020) & LA/P/0006/2020, financed by national funds through the FCT/MCTES (PIDDAC). The NMR spectrometer is part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project Nº 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC). T.N. acknowledges FCT Individual Call to Scientific Employment Stimulus 2022 (2022.02925.CEECIND/CP1728/CT0004, doi: 10.54499/ 2022.02925.CEECIND/CP1728/CT0004). |
Summary of all studies in project PR002132
| Study ID | Study Title | Species | Institute | Analysis (* : Contains Untargted data) |
Release Date | Version | Samples | Download (* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003473 | 1H NMR metabolomics applied to assess the direct and transgenerational effects of simvastatin on the metabolism of the amphipod Gammarus locusta | Gammarus locusta | University of Aveiro | NMR* | 2025-01-12 | 1 | 39 | Uploaded data (33M)* |
