Summary of project PR002148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002148. The data can be accessed directly via it's Project DOI: 10.21228/M8ZR7T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002148 |
| Project DOI: | doi: 10.21228/M8ZR7T |
| Project Title: | Quantifying acyl-chain diversity in isobaric compound lipids containing monomethyl branched-chain fatty acids |
| Project Summary: | To assess whether branched-chain fatty acids (BCFAs) could be effectively separated and quantified using a C30 liquid chromatography column, we treated 3T3-L1 cells with a variety of lipid substrates, including odd-chain, even-chain, and branched-chain fatty acids. This initial set of experiments aimed to determine whether retention times for compound lipids shifted with the incorporation of different substrates. Despite identical lipid masses, a shift in retention time would indicate that compound lipids with monomethyl branched fatty acids could be separated. Furthermore, we engineered 3T3-L1 cells lacking branched-keto acid dehydrogenase A (Bckdha) and validated our findings using a deuterated BCFA (iso-FA 16:0[D7]). We observed incorporation of this deuterated BCFA into compound lipids, accompanied by a corresponding shift in retention time. Lipid species were analyzed via UHPLC-MS, confirming that the C30 column successfully separated monomethyl BCFA-containing lipids from their straight-chain counterparts. |
| Institute: | Salk Institute |
| Last Name: | Kolar |
| First Name: | Matthew |
| Address: | 10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA |
| Email: | mkolar@salk.edu |
| Phone: | 8584534100 |
Summary of all studies in project PR002148
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003502 | Quantifying acyl-chain diversity in isobaric compound lipids containing monomethyl branched-chain fatty acids | Mus musculus | Salk Institute for Biological Studies | MS | 2024-10-18 | 1 | 12 | Uploaded data (636.2M)* |
