Summary of project PR002151
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002151. The data can be accessed directly via it's Project DOI: 10.21228/M8KG00 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002151 |
| Project DOI: | doi: 10.21228/M8KG00 |
| Project Title: | Uncovering the de novo synthesis of polyamines in gut microbiome using stable isotope resolved metabolomics |
| Project Type: | metabolomics |
| Project Summary: | Using 13C-inulin as a tracer, we tracked the biosynthesis of polyamines in the human and mouse fecal microbiome. Additionally, single-strain SIRM analyses was used to explore functional gut microbes. Liquid chromatography-high resolution mass spectrometry analysis revealed distinct 13C enrichment profiles for polyamines. SIRM analyses were performed using a Q-Exactive HF mass spectrometer, equipped with an Ion Max API source and a HESI II probe, and were coupled to a Dionex UltiMate 3000 UHPLC system (Thermo Fisher Scientific). The findings revealed a novel de novo SPD biosynthesis pathway in the human gut microbiome, and Bacteroides (including B.fragilis and B.thetaiotaomicron) contributed to the biosynthesis of polyamines, underscoring the importance of polyamine bioanalysis in aligning gut microbial functions to host intestinal health. |
| Institute: | Soochow University |
| Last Name: | li |
| First Name: | xinwei |
| Address: | 199 Renai Road, Xietang Street, Suzhou, Suzhou, Jiangsu Province, 215031, China |
| Email: | lxw9911117@163.com |
| Phone: | 19971871675 |
Summary of all studies in project PR002151
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003505 | Investigation of polyamine biosynthesis and metabolism in gut microbiome by stale isotope resolved metabolomics | Human (Intestinal bacteria); Mouse (Intestinal bacteria); Bacteroides (B.fragilis and B.thetaiotaomicron) | Soochow University | MS* | 2025-02-11 | 1 | 24 | Uploaded data (3.9G)* |
