Summary of project PR002186
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002186. The data can be accessed directly via it's Project DOI: 10.21228/M82B9C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002186 |
| Project DOI: | doi: 10.21228/M82B9C |
| Project Title: | Stable-isotope lysine tracing to differentiate utilization of the saccharopine and/or pipecolate pathways in HEK293 cells and primary mouse astrocytes. |
| Project Type: | Manuscript |
| Project Summary: | L-lysine (Lysine) is an essential proteinogenic amino acid that is acquired from a protein-rich diet. Lysine is metabolized to fuel acyl-CoA and L-carnitine synthesis in cells and tissues. The two main catabolic pathways, named after their products pipecolate or saccharopine, differ in their cytosolic or mitochondrial localization, respectively, but converge at the mitochondrial enzyme α-aminoadipic semialdehyde dehydrogenase (ALDH7A1, or antiquitin). To differentiate between the utilization of these two pathways, we cultured HEK293 cells or primary mouse astrocytes with unlabeled L-lysine, 13C6-L-lysine, alpha-15N-L-lysine, or epsilon-15N-L-lysine for 24 hours and analyzed atom incorporation into downstream metabolites using LCMS. Our results suggest that both cell types exclusively use the saccharopine pathway. In addition, we show that pipecolate can be generated from interconversion of piperideine 6-carboxylate rather than from activity of the pipecolate pathway, which was silent in both cell types. |
| Institute: | University of British Columbia |
| Department: | Biochemistry & Molecular Biology |
| Laboratory: | Parker laboratory |
| Last Name: | Parker |
| First Name: | Seth |
| Address: | 950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada |
| Email: | seth.parker@bcchr.ca |
| Phone: | 6048753121 |
Summary of all studies in project PR002186
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003550 | Stable-isotope lysine tracing in primary mouse astrocytes and HEK293 cells. | Mouse and Human | University of British Columbia | MS | 2024-11-15 | 1 | 60 | Uploaded data (13.3G)* |
