Summary of project PR002191
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002191. The data can be accessed directly via it's Project DOI: 10.21228/M8DN9K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002191 |
| Project DOI: | doi: 10.21228/M8DN9K |
| Project Title: | N-methyl-arginine is taken up into the liver, brain, and kidney of mice. |
| Project Type: | Manuscript |
| Project Summary: | N-methyl-arginine was identified as a transported substrate through multiple cationic amino acid transporters, which facilitate the uptake of lysine, arginine, and other structurally related amino acids. In cell culture, N-methyl-arginine inhibits the uptake and metabolism of lysine. In this project, we aimed to determine if N-methyl-arginine administered to mice at 80 mg/kg was sufficient to inhibit tissue lysine uptake and metabolism. Following a single injection, N-methyl-arginine significantly accumulated in mouse plasma and tissues but failed to significantly inhibit the level of lysine metabolites (e.g., saccharopine). These results are in-line with in vitro results suggesting that N-methyl-arginine is a relatively low affinity transport inhibitor. |
| Institute: | University of British Columbia |
| Department: | Biochemistry & Molecular Biology |
| Laboratory: | Parker laboratory |
| Last Name: | Parker |
| First Name: | Seth |
| Address: | 950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada |
| Email: | seth.parker@bcchr.ca |
| Phone: | 6048753121 |
Summary of all studies in project PR002191
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003557 | Metabolomics analysis of kidney, brain, liver, and plasma from Aldh7a1-/- mice administered PBS or N-methyl-arginine (80 mg/kg) via a single intraperitoneal injection. | Mus musculus | University of British Columbia | MS | 2024-11-15 | 1 | 39 | Uploaded data (8G)* |
