Summary of project PR002194
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002194. The data can be accessed directly via it's Project DOI: 10.21228/M81C1C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002194 |
| Project DOI: | doi: 10.21228/M81C1C |
| Project Title: | Poplar leaf bud resin metabolomics: Seasonal profiling of leaf bud chemistry in Populus trichocarpa provides insight into resin biosynthesis |
| Project Summary: | Trees in the genus Populus synthesize sticky and fragrant resins to protect dormant leaf buds during winter. These resins contain diverse phenolic metabolites, in particular hydroxycinnamate esters and methylated flavonoids. P. trichocarpa leaf bud resin is characterized by methylated dihydrochalcone aglycones. To determine how the resin profile is influenced by seasonal changes, P. trichocarpa lateral leaf bud extracts (Total_resin_positive_mode.txt) and secreted surface resin (Surface_resin_positive_mode.txt) were collected monthly over a one-year cycle. The dihydrochalcones in both sets of extracts were quantified using UPLC-MS and other chemical changes monitored using nontargeted metabolomics by UPLC-HRMS. The results indicate that the dihydrochalcone content changes over the seasons and that biosynthesis occurs concomitant with bud development in the summer months. Non-targeted metabolomics data confirmed a pattern of dramatic changes in the summer, and further suggested additional periods of substantive biochemical change in the resin. While overall patterns of surface-extracted resin matched that of whole bud extracts, some of the dynamics were shifted in the surface resin samples. This study provides the basis for the use of dihydrochalcones and other identified resin components as metabolic markers for more detailed investigations of resin biosynthesis, secretion and movement to the bud surface. |
| Institute: | University of Victoria |
| Last Name: | Constabel |
| First Name: | C. Peter |
| Address: | 3800 Finnerty Road | Victoria BC | V8P 5C2 Canada |
| Email: | cpc@uvic.ca |
| Phone: | (250) 472-5140 |
| Contributors: | Piirtola, Eerik-Mikael (eerik.piirtola@ubc.ca, Phone: 604 690 0193) |
Summary of all studies in project PR002194
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003560 | Poplar leaf bud resin metabolomics: Seasonal profiling of leaf bud chemistry in Populus trichocarpa provides insight into resin biosynthesis | Populus trichocarpa | University of Victoria | MS* | 2024-12-02 | 1 | 191 | Uploaded data (4G)* |
