Summary of project PR002198
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002198. The data can be accessed directly via it's Project DOI: 10.21228/M8HC23 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002198 |
| Project DOI: | doi: 10.21228/M8HC23 |
| Project Title: | Metabolomics of Papanicolaou Tests for the Discovery of Ovarian Cancer Biomarkers |
| Project Type: | Untargeted Metabolomics |
| Project Summary: | Ovarian cancer (OC) remains one of the most lethal cancers among female populations due to the vast majority of cases going undiagnosed until later stages (stage III, IV). Early detection and treatment of this malignancy provides the best prognosis, but the lack of accurate and sensitive screening tools combined with the presence of ambiguous symptoms hinders these diagnoses. In contrast, screening for cervical cancer via Papanicolaou (Pap) tests is a widespread practice with use spanning the past several decades, reducing this cancer’s morbidity and mortality rates. Two types of Pap smear tests exist: a conventional method where cells from the ectocervix are stained on a glass slide and a liquid-based cytology method. Interestingly, previous studies show evidence of OC cells in Pap tests, suggesting that lipids shed from ovarian tumors may end up in the cervix. The goal of this study is to evaluate the practicality of using liquid-based Pap tests as biospecimens for OC screening-related metabolic studies. Cell pellets from liquid-based Pap tests from 29 healthy women were analyzed via ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). This approach facilitated the detection and annotation of 453 unique lipids across 20 lipid subclasses, including ceramides, triacylglycerols, and hexosylceramides. These results demonstrated the feasibility of using an MS-based approach to analyze residual Pap test samples for the discovery of OC-related lipid biomarkers with the goal of detecting OC at early stages of the disease. |
| Institute: | Georgia Institute of Technology |
| Department: | Chemistry and Biochemistry |
| Laboratory: | Fernandez Lab |
| Last Name: | Schwiebert |
| First Name: | Elisabeth |
| Address: | 311 Ferst Dr NW, Atlanta, Georgia, 30318, USA |
| Email: | eschwiebert3@gatech.edu |
| Phone: | 4043854432 |
| Funding Source: | National Institutes of Health's National Center for Advancing Translational Sciences |
Summary of all studies in project PR002198
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003564 | Metabolomics of Papanicolaou Tests for the Discovery of Ovarian Cancer Biomarkers | Homo sapiens | Georgia Institute of Technology | MS* | 2024-11-27 | 1 | 46 | Uploaded data (25.6G)* |
