Summary of project PR002201
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002201. The data can be accessed directly via it's Project DOI: 10.21228/M84517 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002201 |
| Project DOI: | doi: 10.21228/M84517 |
| Project Title: | Analysis of maize Anther Wax, Cutin, and total fatty acids (TFA) of WT and enr1 mutant |
| Project Type: | Detection of small molecular compounds in cutin and wax. Detection of free fatty acids and dissociated long-chain fatty acids in TFA. |
| Project Summary: | Lipid metabolism is very important for plant male reproduction. Many lipid metabolism genes, male sterility (GMS) genes play a role in the endoplasmic reticulum of anther tapetum, but little is known about GMS genes involved in the de novo synthesis of fatty acids in anther tapetum. Using gene mapping, we cloned a new GMS gene, enoyl-acyl carrier protein (ACP) reductase (ZmENR1). enr1 (mutation of enoyl-ACP reductase gene) has early tapetum degradation, anther epidermis and pollen exine defects. In order to further study the function of ZmENR1, we compared the changes of lipid content in anthers of wild-type (WT) and enr1 mutants at maturity. The expression of ZmENR1 significantly affected the contents of cutin, wax and total fatty acids (TFA) in anthers. In addition, enr1 mutant reduced the expression of cutin/wax and sporopollen related genes to inhibit the formation of anther epidermis and pollen exine. Therefore, our researches provide a new insight into the synthesis of fatty acids mediated by ZmENR1, and provide an insight into the molecular mechanism of ZmENR1 regulating maize male sterility. |
| Institute: | University of Science and Technology Beijing |
| Last Name: | Zhang |
| First Name: | Shaowei |
| Address: | 30 XUEYUAN ROAD, HAIDIAN DISTRICT, BEIJING 100083, CHINA |
| Email: | b20200413@xs.ustb.edu.cn |
| Phone: | 0086-13271969211 |
Summary of all studies in project PR002201
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003567 | Enoyl-acyl carrier protein reductase (ZmENR1) expression level is critical for de novo fatty acid biosynthesis and anther cuticle and pollen exine formation in maize. | Zea mays | University of Science and Technology Beijing | MS | 2024-11-12 | 1 | 21 | Uploaded data (67.9M)* |
