Summary of project PR002285
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002285. The data can be accessed directly via it's Project DOI: 10.21228/M88G2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002285 |
| Project DOI: | doi: 10.21228/M88G2Z |
| Project Title: | Adenosine Deaminase function in C. elegans |
| Project Summary: | We have previously demonstrated that the C. elegans pnp-1 gene encodes a purine nucleoside phosphorylase that catalyzes the conversion of inosine to hypoxanthine. We hypothesize that the C. elegans adah-1 gene encodes the adenosine deaminase that acts upstream of PNP-1 to produce inosine from adenosine. To investigate the hypothesis that ADAH-1 functions as a canonical adenosine deaminase, we used RNAi to knockdown expression of ADAH-1, and used LC-MS to compare relative levels of adenosine, inosine, and hypoxanthine in the knockdown animals and an RNAi control (L4440). As a control we also confirmed the activity of pnp-1, by comparing levels of adenosine, inosine, and hypoxanthine in control (N2) and the loss of function mutant pnp-1(jy90). As expected we found that levels of hypoxanthine were reduced and levels of inosine were increased in pnp-1 mutants relative to control. In contrast, RNAi against adah-1 led to significantly increased levels of adenosine, and decreased levels of inosine and the downstream metabolite hypoxanthine. We conclude that ADAH-1 is a bona fide adenosine deaminase. |
| Institute: | Pennsylvania State University |
| Department: | Biochemistry and Molecular Biology |
| Last Name: | Hanna-Rose |
| First Name: | Wendy |
| Address: | 432 Science Drive, LSB Room 104D |
| Email: | wxh21@psu.edu |
| Phone: | 8149337569 |
Summary of all studies in project PR002285
| Study ID | Study Title | Species | Institute | Analysis (* : Contains Untargted data) |
Release Date | Version | Samples | Download (* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003685 | Investigation of C. elegans adah-1 gene for potential adenosine deaminase activity via quantification of predicted substrate and product in control animals and animals with knockdown in expression of adah-1 | Caenorhabditis elegans | Pennsylvania State University | MS | 2025-02-14 | 1 | 38 | Uploaded data (20.3G)* |
