Summary of project PR002294
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002294. The data can be accessed directly via it's Project DOI: 10.21228/M83R72 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002294 |
| Project DOI: | doi: 10.21228/M83R72 |
| Project Title: | Adaptations to virulence and growth of Brucella abortus envelope |
| Project Summary: | Bacteria of the Brucella genus are collectively responsible for brucellosis, a widely spread zoonosis. Our laboratory developed molecular tools to study growth and virulence of Brucella abortus at the single cell level. Using a genetic approach, we found that B. abortus selected a hybrid Mla-Pqi system (here called Mpc) anchored in the envelope, that is necessary to resist to envelope stress and to infect macrophages. We also found that the main component of the outer membrane, the lipopolysaccharide, is displaying a surprising traffic inside bacteria (the “round trip model”) that we propose to investigate. We also constructed a strain (here called RgsE*) that is growing bipolarly instead of unipolarly and we propose to analyze it to better understand polar ageing and potential polar virulence factors. One such polar virulence factor is the type 4 secretion system VirB, that we found associated to the new pole of the bacterium during macrophage infection. Altogether, our project aims to get a better understanding of envelope growth and properties, especially related to the virulence of B. abortus, with a high potential to generate new concepts that could be applicable to other bacterial pathogens. |
| Institute: | Université de Namur |
| Last Name: | de Bolle |
| First Name: | Xavier |
| Address: | Rue de Bruxelles 61, 5000 Namur, Bélgica |
| Email: | xavier.debolle@unamur.be |
| Phone: | 690090778 |
Summary of all studies in project PR002294
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003696 | A lipid transport Mla Pqi Chimeric system is essential for Brucella abortus survival in macrophages | Brucella abortus | CEMBIO | MS* | 2025-06-20 | 1 | 24 | Uploaded data (1.7G)* |
