Summary of project PR002295
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002295. The data can be accessed directly via it's Project DOI: 10.21228/M8ZZ54 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002295 |
| Project DOI: | doi: 10.21228/M8ZZ54 |
| Project Title: | Impact of High Fat diet-induced MASLD on Heart, Kidney and Skeletal Muscle Metabolomes in Wild-type Mice |
| Project Type: | 1H NMR metabolomics to study the effects high-fat diet-induced MASLD on extrahepatic tissues metabolomes, namely the heart, kidney and skeletal muscle, in C57BL6J wild-type mice. |
| Project Summary: | Excessive caloric intake is a primary driver of metabolic dysfunction-associated steatotic liver disease (MASLD), and this has been recapitulated in mice fed a high-fat diet. In 2023, the global prevalence of MASLD was estimated at 30%, with high incidences affecting wealthy urbanised countries. This implication of hypercaloric diets can also perturb metabolism and function of extrahepatic tissues such as heart, kidney and skeletal muscle. These effects that can take place in extrahepatic tissues are still poorly understood in terms of metabolic alterations and physiology, and represent an important point of improvement in the knowledge gap that connects early stage MASLD with other obesity related comorbidities, such as type 2 diabetes, insulin resistance, cardiovascular and renal complications, and overall, with the so known metabolic syndrome. In this study, we aimed to evaluate the potential of using metabolomics to unravel the effects and interactions taking place in a diet-induced MASLD model related to the development of the disorder. Black-6 mice were subjected to either a control diet or a high-fat diet for 18 weeks, from which at the end their heart, kidney and skeletal muscle metabolites were extracted. The metabolites, divided into aqueous and lipophilic fractions, were acquired by 1H-NMR, and then processed using a untargeted Metabolomics and Lipidomics analysis approach, to identify key changes occurring between control and high-fat diet in these models. These results added important information to better understand the link between early onset MASLD and the Metabolic Syndrome and its comorbidities, though several metabolic changes in the extrahepatic tissues, namely in ectopic fat deposition and alterations to Randle cycle and gut microbiota activity. |
| Institute: | Center for Innovative Biomedicine and Biotechnology (CIBB UC) |
| Department: | Institute of Interdisciplinary Research |
| Last Name: | Silva |
| First Name: | João |
| Address: | Rua Larga - Faculdade de Medicina, 1ºandar - POLO I Universidade de Coimbra |
| Email: | jgsilva@cnc.uc.pt |
| Phone: | (+351) 239 820 190 |
| Project Comments: | Full NMR sample preparation and analysis procedures are available in the accompanying document entitled 1. MASLD Extrahepatic Metabolomics experimental procedure. The normalized data that was used in uni- and multivariate analysis is available in the accompanying files: 4. MASLD Extrahepatic Metabolomics results data.txt The raw fid as well as 1r file can be found in 5. MASLD Extrahepatic Metabolomics 1H NMR Raw Data.zip |
Summary of all studies in project PR002295
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003698 | Impact of High Fat diet-induced metabolic dysfunction-associated steatotic liver disease (MASLD) on Heart, Kidney and Skeletal Muscle Metabolomes in Wild-type Mice | Mus musculus | Center for Innovative Biomedicine and Biotechnology (CIBB UC) | NMR* | 2025-02-03 | 1 | 131 | Uploaded data (59.5M)* |
