Summary of project PR002296
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002296. The data can be accessed directly via it's Project DOI: 10.21228/M8V83J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002296 |
| Project DOI: | doi: 10.21228/M8V83J |
| Project Title: | Perturbed pediatric circulating metabolome in mild and severe dengue disease |
| Project Summary: | Four billion people are at risk of infection with dengue viruses (DENV), and this burden is rapidly increasing due to geographic expansion of the mosquito vector. Infection with any of the four serotypes of DENV can result in a self-limiting but debilitating febrile illness (DF), and some infections progress to severe disease with hemorrhagic manifestations and shock (dengue hemorrhagic fever/dengue shock syndrome [DHF/DSS]). DENV infection drives the metabolic state of host cells for viral benefit and induces a host-immune response with metabolic implications that link to disease. Here, a dynamic metabolic response to DENV infection and disease was measured in 535 pediatric patients from Nicaragua using liquid chromatography-tandem mass spectrometry. Metabolomic analyses revealed profound disruptions of critical biochemical pathways and metabolites within the circulating metabolome, especially in those with more severe manifestations of dengue disease. A biomarker panel of 28 metabolites was utilized to classify DF versus DHF/DSS with high sensitivity and specificity, equating to a balanced accuracy of 96.88%. Structurally confirmed metabolites belonged to biochemical pathways of omega-3 and omega-6 fatty acids, sphingolipids, dipeptides, purines, and tryptophan metabolism. Dipeptides emerged as the most critical molecules for severe disease classification. Additionally, a previously reported trend between serotonin and platelets in DHF patients was expanded upon here, revealing a major depletion of serotonin, but not platelets, in DSS patients. In this study, the perturbed metabolome was used for disease state classification and exploration of the biochemistry of severe dengue disease pathology. |
| Institute: | Colorado State University |
| Department: | Microbiology, Immunology, Pathology |
| Laboratory: | Perera |
| Last Name: | Soma |
| First Name: | Paul |
| Address: | 3185 Rampart Rd, Foothills Campus, Fort Collins, CO 80526 |
| Email: | paul.soma@colostate.edu |
| Phone: | 610-613-6772 |
Summary of all studies in project PR002296
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003699 | Perturbed pediatric circulating metabolome in mild and severe dengue disease | Homo sapiens | Colorado State University | MS* | 2025-09-29 | 1 | 535 | Uploaded data (22G)* |
