Summary of project PR002330
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002330. The data can be accessed directly via it's Project DOI: 10.21228/M8FV76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002330 |
| Project DOI: | doi: 10.21228/M8FV76 |
| Project Title: | A study exploring the epoxidation function of ODD enzymes (2-oxoglutarate/Fe(II)-dependent dioxygenases) |
| Project Type: | MS Qualitative Analysis |
| Project Summary: | This study identified a series of ODD (2-oxoglutarate/Fe(II)-dependent dioxygenase) genes with potential C4β-C20 epoxidation activity. To validate their enzymatic function, these genes were cloned into the Ycplac22 vector and introduced into Saccharomyces cerevisiae engineered to produce the necessary ODD substrate, taxadiene. The fermentation products were then analyzed using GC-MS to detect the formation of the target compound, 4β,20-taxadiene. |
| Institute: | Northwestern Polytechnical University |
| Last Name: | Li |
| First Name: | Zhenzhu |
| Address: | No. 127, Youyi West Road, Xi'an City |
| Email: | lizhenzhu@mail.nwpu.edu.cn |
| Phone: | 18829012174 |
Summary of all studies in project PR002330
| Study ID | Study Title | Species | Institute | Analysis (* : Contains Untargted data) |
Release Date | Version | Samples | Download (* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003744 | A study exploring the epoxidation function of ODD enzymes (2-oxoglutarate/Fe(II)-dependent dioxygenases) | Taxus wallichiana | Northwestern Polytechnical University | MS | 2025-02-24 | 1 | 12 | Uploaded data (719.3M)* |
