Summary of project PR002350
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002350. The data can be accessed directly via it's Project DOI: 10.21228/M8VV7X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002350 |
| Project DOI: | doi: 10.21228/M8VV7X |
| Project Title: | N-acetylneuraminic Acid may play Links Secondary Neurodegeneration and Cognitive Impairment in Cortical Photothrombotic Stroke Mouse Model |
| Project Summary: | ABSTRACT Background: Post-stroke cognitive impairment (PSCI) is a major sequelae of ischemic stroke (IS), but the associated mechanisms remain unclear. Recent studies have shown that cortical stroke can cause distal brain disturbances, leading to cognitive decline. This study aims to investigate the neuropathological and metabolic changes over time in the remote brain region associated with cognitive impairment after cortical stroke. Methods: We assessed cognitive function in photothrombotic (PT) mice for 84 days and identified distal brain regions with secondary neurodegeneration (SND) using voxel-based morphometry (VBM). Integrated untargeted and targeted metabolomics method was established to comprehensively analyze the molecular mechanism of SND in this brain region and screen the potential predictive biomarker of PSCI. Furthermore, the mechanism of the biomarker with PSCI and SND was studied in vitro. Results: The results show that recent memory impairment, remote dysfunction, and anxiety persisted for 84 days in PT mice. The hippocampus of cognitively impaired PT mice developed SND and metabolic disorders, particularly oxidative stress, lipid peroxidation, and inflammation. N-acetylneuramic acid (Neu5Ac) was screened in the hippocampus of mice, serum of mice and 154 IS patients. Neu5Ac promotes oxidative stress and inflammation in microglia. Conclusions: Our results suggest that hippocampal SND is closely related to cognitive impairment in PT mice, while oxidative stress and inflammation are important factors in hippocampal SND. Neu5Ac may aggravate PSCI by causing hippocampal SND through inducting of microglia-driven oxidative stress and inflammation, and it may thus be a potential predictive biomarker of PSCI. |
| Institute: | Wenzhou Medical University |
| Last Name: | Zhou |
| First Name: | Yiyang |
| Address: | University Town, Chashan, Wenzhou, Zhejiang, 325035 P.R China |
| Email: | zhouyiyang@wmu.edu.cn |
| Phone: | +8613806831161 |
Summary of all studies in project PR002350
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003767 | N-acetylneuraminic Acid may play Links Secondary Neurodegeneration and Cognitive Impairment in Cortical Photothrombotic Stroke Mouse Model | Mus musculus | Wenzhou Medical University | MS* | 2025-06-24 | 1 | 54 | Uploaded data (8.1G)* |
