Summary of project PR002391
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002391. The data can be accessed directly via it's Project DOI: 10.21228/M8K82P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002391 |
| Project DOI: | doi: 10.21228/M8K82P |
| Project Title: | An integrated analysis of behavioral and metabolic modulations under a chronobiotic treatment in Drosophila melanogaster |
| Project Type: | MS based Metabolomics analysis |
| Project Summary: | Drosophila melanogaster, with its conserved molecular clocks and accessible assays, serves as an ideal model system for studying circadian rhythms and sleep. Using an integrated approach combining high-throughput locomotor activity monitoring and untargeted metabolomics, we analyzed the behavioral and metabolic effects of a chronobiotic melatonin. The behavioral activity of flies was recorded using an infrared-based monitoring device followed by data analysis with open-source data packages, ShinyR-DAM and VANESSA. We found that 1mM and 4mM melatonin doses significantly increased Drosophila locomotor activity. Melatonin at high concentrations (4mM) exhibited protective effects by reducing mortality in Drosophila. Despite these changes, melatonin preserved the flies; endogenous bimodal activity pattern, maintaining circadian alignment. High- performance LC-MS metabolomics identified differentially abundant metabolites under drug treatment. LC-MS-based metabolomics identified 20 biologically relevant metabolites altered by melatonin, including key perturbations in arginine biosynthesis, alanine/aspartate/glutamate metabolism, and pyrimidine pathways. Notably, melatonin upregulated glutamine, a potential indicator of enhanced neurotransmitter synthesis and broadly modulated amino acid and nucleotide metabolism, suggesting dual roles in neuroprotection and energy homeostasis. This high-throughput approach uncovers melatonin- induced behavioral and metabolic perturbations, revealing how melatonin modulates locomotor activity, survival, and circadian integrity through specific alterations in metabolism. |
| Institute: | Indian Institute of Technology Hyderabad |
| Department: | Biotechnology |
| Laboratory: | Circadian Rhythms and Disease Biology Laboratory |
| Last Name: | Das |
| First Name: | Sandip |
| Address: | NH-9,KANDI, sangareddy, telengana, 502285, India |
| Email: | bo21resch11009@iith.ac.in |
| Phone: | 6290857805 |
Summary of all studies in project PR002391
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003828 | An integrated analysis of behavioral and metabolic modulations under a chronobiotic treatment in Drosophila melanogaster | Drosophila melanogaster | Indian Institute of Technology Hyderabad | MS | 2025-05-28 | 1 | 18 | Uploaded data (10.3G)* |
