Summary of project PR002418
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002418. The data can be accessed directly via it's Project DOI: 10.21228/M83542 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002418 |
| Project DOI: | doi: 10.21228/M83542 |
| Project Title: | Comparative effects of nano urea and equimolar urea on the metabolome profile of Arabidopsis thaliana seedlings |
| Project Type: | GC MS |
| Project Summary: | We compared the effects of Indian Farmers Fertilizer Cooperative (IFFCO) nano urea formulation with equimolar conventional urea on the metabolome of Arabidopsis thaliana seedlings. Seedlings were grown in hydroponics in 1/4th strength Hoagland's solution for 12 days, then transferred to a nitrogen-free modified Hoagland's solution and grown for 21 days. Thereafter whole seedlings were crushed in lquid nitrogen to a fine powder. To 500 mg of fine powder, 5 mL of HPLC-grade methanol was added, and the sample was shaken/vortexed for over 30 minutes to extract analytes. The sample was spun at 8,000 rpm for 10 minutes to separate the liquid phase, which was then transferred to a fresh tube and stored at -20°C. Derivatization was not done, as it was not required for non-volatile compounds. Gas Chromatography-Mass Spectrometry was conducted using GC-MS model QP2010 (Shimadzu®) using a SH-I-5Sil MS Capillary (30 m x 0.25 mm x 0.25 µm) column. 2 µL of samples were injected using a splitless injection mode. Helium (1 mL/min) was used as a carrier gas. Ionization of 70 eV and a running time of 9.10 min – 52.0 min was used. The oven Program included an initial temperature of 45°C for 2 min, ramped to 140°C at 5°C/min, and then increased to 280°C, held isothermally for 10 min. The NIST14.L library (2020) was used to identify compounds based on retention times and mass spectra. |
| Institute: | Indian Institute of Technology Jodhpur |
| Department: | BIOSCIENCE AND BIOENGINEERING |
| Laboratory: | Plant functional genomics |
| Last Name: | SADHUKHAN |
| First Name: | AYAN |
| Address: | NH62, NAGAUR ROAD |
| Email: | ayansadhukhan@iitj.ac.in |
| Phone: | 09038013015 |
| Funding Source: | IFFCO |
Summary of all studies in project PR002418
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003861 | Comparative effects of nano urea and equimolar urea on the metabolome profile of Arabidopsis thaliana seedlings | Arabidopsis thaliana | Indian Institute of Technology Jodhpur | MS | 2025-06-24 | 1 | 9 | Uploaded data (171.1M)* |
